Suda H, Moroi C, Inada K, Chida S, Fujiwara T, Yoshida M
Department of Neonatology, Morioka Red Cross Hospital, Japan.
Acta Paediatr Jpn. 1995 Oct;37(5):579-81. doi: 10.1111/j.1442-200x.1995.tb03380.x.
The endotoxin in blood was measured to establish both the cut-off value and to detect Gram-negative septicemia. We employed a new perchloric acid treatment method using an endotoxin-specific chromogenic Limulus test (Endospecy test). The cut-off value of endotoxin in blood was 11.2 pg/mL. All cases of septicemia (n = 7) showed high values of endotoxin. Three cases were Group B streptococci, and two cases were Escherichia coli. The others were showed to be negative in blood cultures. The paired values of endotoxin titers during a 48 h interval were useful to evaluate the effectiveness of antibiotics.
检测血液中的内毒素以确定临界值并检测革兰氏阴性菌败血症。我们采用了一种新的高氯酸处理方法，使用内毒素特异性显色鲎试剂试验（Endospecy试验）。血液中内毒素的临界值为11.2 pg/mL。所有败血症病例（n = 7）的内毒素值都很高。3例为B组链球菌，2例为大肠杆菌。其他病例血培养显示为阴性。48小时内毒素滴度的配对值有助于评估抗生素的疗效。
