[Influence of testosterone on the distribution of 65Zn-binding proteins in the prostate and seminal vesicles of rats (author's transl)].

65Zn (7.4 MBq: 200 muCi) was injected intravenously into normal, castrated and castrated, testosterone-substituted rats. After 1, 24 and 48 hours, the distribution of 65Zn-binding proteins in the 100,000 g supernatant of the prostate and seminal vesicles was investigated by separation on Sephadex G 100. The prostate and seminal vesicles from any one rat showed the same distribution pattern of 65Zn-proteins. In castrated rats, the incorporation of 65Zn was, however, 5-6 times lower than in the normal or castrated, testosterone-substituted rats. One hour after the injection, the highest activity of 65Zn was found in proteins in the molecular weight range above 100,000. After 48 hours the greatest proportion of 65Zn was present peak corresponding to 28,000 Daltons.