Arlt R, Förster R, Scherr F, Günther T
J Clin Chem Clin Biochem. 1977 Jul;15(7):481-4.
65Zn (7.4 MBq: 200 muCi) was injected intravenously into normal, castrated and castrated, testosterone-substituted rats. After 1, 24 and 48 hours, the distribution of 65Zn-binding proteins in the 100,000 g supernatant of the prostate and seminal vesicles was investigated by separation on Sephadex G 100. The prostate and seminal vesicles from any one rat showed the same distribution pattern of 65Zn-proteins. In castrated rats, the incorporation of 65Zn was, however, 5-6 times lower than in the normal or castrated, testosterone-substituted rats. One hour after the injection, the highest activity of 65Zn was found in proteins in the molecular weight range above 100,000. After 48 hours the greatest proportion of 65Zn was present peak corresponding to 28,000 Daltons.
将65锌（7.4兆贝可：200微居里）静脉注射到正常、去势以及去势后用睾酮替代的大鼠体内。在1小时、24小时和48小时后，通过在葡聚糖G 100上进行分离，研究前列腺和精囊100,000克上清液中65锌结合蛋白的分布情况。来自任何一只大鼠的前列腺和精囊显示出相同的65锌蛋白分布模式。然而，在去势大鼠中，65锌的掺入量比正常或去势后用睾酮替代的大鼠低5至6倍。注射后1小时，在分子量高于100,000的蛋白质中发现65锌的活性最高。48小时后，65锌的最大比例存在于对应于28,000道尔顿的峰中。
