Hara T, Hoshi N, Hara T
Hara Eye Hospital, Utsunomiya, Japan.
Ophthalmology. 1996 Nov;103(11):1876-9. doi: 10.1016/s0161-6420(96)30413-2.
To investigate the rate of contamination of conjunctival smears and anterior chamber aspirates at the conclusion of cataract surgery, and the relation between the types of bacterial strain in the conjunctival sac and anterior chamber at the conclusion of surgery and in preoperative samples from the conjunctival sac of the same eye.
The bacterial strains in conjunctival smears 1 week preoperatively and in conjunctival smears and anterior chamber aspirates at the conclusion of surgery from 58 consecutive eyes of 48 patients were examined. The patients underwent cataract surgery by phacoemulsification and aspiration followed by intraocular lens (IOL) implantation. All surgeries were broadcast simultaneously by closed-circuit television to the patients' families who were in a separate waiting room.
At the conclusion of surgery, six eyes (10.3%) were contaminated: one anterior chamber aspirate (1.7%) and five conjunctival smears (8.6%). Only two (33%) of the six contaminated eyes at the conclusion of surgery had the same bacterial strain as the preoperative conjunctival smears.
The percentage of culture-positive anterior chamber aspirates at the conclusion of phacoemulsification surgery and intraocular lens implantation was only 1.7%, despite a concurrent televised broadcast accompanied by an intraoperative microphone-transmitted explanation by the surgeon. Sixty-seven percent of the bacterial strains at the conclusion of surgery did not match those found preoperatively in the same eye.
研究白内障手术结束时结膜涂片和前房穿刺液的污染率，以及手术结束时结膜囊和前房内细菌菌株类型与同眼术前结膜囊样本中细菌菌株类型之间的关系。
对48例患者的58只连续眼睛术前1周的结膜涂片以及手术结束时的结膜涂片和前房穿刺液中的细菌菌株进行检测。患者接受白内障超声乳化吸除术并植入人工晶状体。所有手术均通过闭路电视同步播放给在单独候诊室的患者家属。
手术结束时，6只眼（10.3%）被污染：1只前房穿刺液（1.7%）和5只结膜涂片（8.6%）。手术结束时6只被污染眼中只有2只（33%）的细菌菌株与术前结膜涂片相同。
尽管在手术过程中有电视转播且外科医生通过术中麦克风进行解说，但在白内障超声乳化吸除术和人工晶状体植入术结束时，培养阳性的前房穿刺液比例仅为1.7%。手术结束时67%的细菌菌株与同眼术前发现的菌株不匹配。
