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本文引用的文献

1
Micromachining a miniaturized capillary electrophoresis-based chemical analysis system on a chip.在芯片上微加工基于毛细管电泳的微型化学分析系统。
Science. 1993 Aug 13;261(5123):895-7. doi: 10.1126/science.261.5123.895.
2
Accessing genetic information with high-density DNA arrays.使用高密度DNA阵列获取遗传信息。
Science. 1996 Oct 25;274(5287):610-4. doi: 10.1126/science.274.5287.610.
3
Cystic fibrosis mutation detection by hybridization to light-generated DNA probe arrays.通过与光生成的DNA探针阵列杂交检测囊性纤维化突变
Hum Mutat. 1996;7(3):244-55. doi: 10.1002/(SICI)1098-1004(1996)7:3<244::AID-HUMU9>3.0.CO;2-A.
4
Extensive polymorphisms observed in HIV-1 clade B protease gene using high-density oligonucleotide arrays.使用高密度寡核苷酸阵列在HIV-1 B亚型蛋白酶基因中观察到广泛的多态性。
Nat Med. 1996 Jul;2(7):753-9. doi: 10.1038/nm0796-753.
5
Mapping genomic library clones using oligonucleotide arrays.使用寡核苷酸阵列对基因组文库克隆进行定位
Genomics. 1996 May 1;33(3):445-56. doi: 10.1006/geno.1996.0219.
6
Microfabricated structures for integrated DNA analysis.
Proc Natl Acad Sci U S A. 1996 May 28;93(11):5556-61. doi: 10.1073/pnas.93.11.5556.
7
Light-generated oligonucleotide arrays for rapid DNA sequence analysis.用于快速DNA序列分析的光生成寡核苷酸阵列。
Proc Natl Acad Sci U S A. 1994 May 24;91(11):5022-6. doi: 10.1073/pnas.91.11.5022.
8
Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips.使用微制造毛细管阵列电泳芯片进行超高速DNA片段分离
Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11348-52. doi: 10.1073/pnas.91.24.11348.
9
A review of microfabricated devices for gene-based diagnostics.
Hematol Pathol. 1995;9(1):1-15.
10
Quantitative monitoring of gene expression patterns with a complementary DNA microarray.利用互补DNA微阵列对基因表达模式进行定量监测。
Science. 1995 Oct 20;270(5235):467-70. doi: 10.1126/science.270.5235.467.

使用半导体光刻胶进行光导向的高密度寡核苷酸阵列合成。

Light-directed synthesis of high-density oligonucleotide arrays using semiconductor photoresists.

作者信息

McGall G, Labadie J, Brock P, Wallraff G, Nguyen T, Hinsberg W

机构信息

Affymetrix, Inc., Santa Clara, CA 95051, USA.

出版信息

Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13555-60. doi: 10.1073/pnas.93.24.13555.

DOI:10.1073/pnas.93.24.13555
PMID:8942972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC19343/
Abstract

High-density arrays of oligonucleotide probes are proving to be powerful new tools for large-scale DNA and RNA sequence analysis. A method for constructing these arrays, using light-directed DNA synthesis with photo-activatable monomers, can currently achieve densities on the order of 10(6) sequences/cm2. One of the challenges facing this technology is to further increase the volume, complexity, and density of sequence information encoded in these arrays. Here we demonstrate a new approach for synthesizing DNA probe arrays that combines standard solid-phase oligonucleotide synthesis with polymeric photoresist films serving as the photoimageable component. This opens the way to exploiting high-resolution imaging materials and processes from the microelectronics industry for the fabrication of DNA probe arrays with substantially higher densities than are currently available.

摘要

高密度寡核苷酸探针阵列正成为大规模DNA和RNA序列分析的强大新工具。一种利用光可激活单体进行光导向DNA合成来构建这些阵列的方法，目前可实现约10(6)个序列/平方厘米的密度。该技术面临的挑战之一是进一步增加这些阵列中编码的序列信息的容量、复杂性和密度。在此，我们展示了一种合成DNA探针阵列的新方法，该方法将标准固相寡核苷酸合成与用作光成像组件的聚合物光刻胶膜相结合。这为利用微电子行业的高分辨率成像材料和工艺来制造密度远高于现有水平的DNA探针阵列开辟了道路。