使用半导体光刻胶进行光导向的高密度寡核苷酸阵列合成。
Light-directed synthesis of high-density oligonucleotide arrays using semiconductor photoresists.
作者信息
McGall G, Labadie J, Brock P, Wallraff G, Nguyen T, Hinsberg W
机构信息
Affymetrix, Inc., Santa Clara, CA 95051, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13555-60. doi: 10.1073/pnas.93.24.13555.
Abstract
High-density arrays of oligonucleotide probes are proving to be powerful new tools for large-scale DNA and RNA sequence analysis. A method for constructing these arrays, using light-directed DNA synthesis with photo-activatable monomers, can currently achieve densities on the order of 10(6) sequences/cm2. One of the challenges facing this technology is to further increase the volume, complexity, and density of sequence information encoded in these arrays. Here we demonstrate a new approach for synthesizing DNA probe arrays that combines standard solid-phase oligonucleotide synthesis with polymeric photoresist films serving as the photoimageable component. This opens the way to exploiting high-resolution imaging materials and processes from the microelectronics industry for the fabrication of DNA probe arrays with substantially higher densities than are currently available.
摘要
高密度寡核苷酸探针阵列正成为大规模DNA和RNA序列分析的强大新工具。一种利用光可激活单体进行光导向DNA合成来构建这些阵列的方法，目前可实现约10(6)个序列/平方厘米的密度。该技术面临的挑战之一是进一步增加这些阵列中编码的序列信息的容量、复杂性和密度。在此，我们展示了一种合成DNA探针阵列的新方法，该方法将标准固相寡核苷酸合成与用作光成像组件的聚合物光刻胶膜相结合。这为利用微电子行业的高分辨率成像材料和工艺来制造密度远高于现有水平的DNA探针阵列开辟了道路。
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