YY1转录起始因子：蛋白质相互作用以及与含有未配对链的DNA位点的结合
YY1 transcriptional initiator: protein interactions and association with a DNA site containing unpaired strands.
作者信息
Usheva A, Shenk T
机构信息
Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, NJ 08544-1014, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13571-6. doi: 10.1073/pnas.93.24.13571.
Abstract
The Ying-Yang 1 protein (YY1) DNA-binding site functions as an initiator element at which YY1, transcription factor IIB (TFIIB), and RNA polymerase II sponsor basal transcription from a supercoiled DNA template. We show that TFIIB binds to YY1, stabilizing its interaction with DNA, and YY1 contacts the large subunit of polymerase II, directing it to the initiation site. YY1 directs initiation from linear DNA containing mismatched sequences within its binding site, leading us to infer that supercoiling facilitates the separation of DNA strands and to suggest that YY1 likely remains bound to the start site as DNA strands separate during initiation. These results provide a mechanistic basis for transcriptional initiation directed by YY1 in the absence of the TATA box-binding protein.
摘要
阴阳1蛋白（YY1）的DNA结合位点起着起始元件的作用，在该位点，YY1、转录因子IIB（TFIIB）和RNA聚合酶II促进从超螺旋DNA模板进行基础转录。我们发现TFIIB与YY1结合，稳定其与DNA的相互作用，并且YY1与聚合酶II的大亚基接触，将其引导至起始位点。YY1指导从其结合位点内含有错配序列的线性DNA起始转录，这使我们推断超螺旋有助于DNA链的分离，并表明在起始过程中当DNA链分离时YY1可能仍与起始位点结合。这些结果为在没有TATA框结合蛋白的情况下由YY1指导的转录起始提供了机制基础。
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本文引用的文献
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Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13577-82. doi: 10.1073/pnas.93.24.13577.
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