Hodge M R, Chun H J, Rengarajan J, Alt A, Lieberson R, Glimcher L H
Department of Cancer Biology, Harvard School of Public Health and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
Science. 1996 Dec 13;274(5294):1903-5. doi: 10.1126/science.274.5294.1903.
The induction of cytokine gene transcription is mediated in part by the nuclear factor of activated T cells (NF-AT). Factors involved in the mechanisms of NF-AT-mediated transcription are not well understood. A nuclear factor that interacted with the Rel homology domain (RHD) of NF-ATp was identified with the use of a two-hybrid interaction trap. Designated NIP45 (NF-AT interacting protein), it has minimal similarity to any known genes. Transcripts encoding this factor were enriched in lymphoid tissues and testes. NIP45 synergized with NF-ATp and the proto-oncogene c-Maf to activate the interleukin-4 (IL-4) cytokine promoter; transient overexpression of NIP45 with NF-ATp and c-maf in B lymphoma cells induced measurable endogenous IL-4 protein production. The identification of NIP45 advances our understanding of gene activation of cytokines, critical mediators of the immune response.
细胞因子基因转录的诱导部分是由活化T细胞核因子（NF-AT）介导的。参与NF-AT介导转录机制的因子尚未完全清楚。利用双杂交相互作用陷阱鉴定出一种与NF-ATp的Rel同源结构域（RHD）相互作用的核因子。它被命名为NIP45（NF-AT相互作用蛋白），与任何已知基因的相似性极小。编码该因子的转录本在淋巴组织和睾丸中富集。NIP45与NF-ATp和原癌基因c-Maf协同作用，激活白细胞介素-4（IL-4）细胞因子启动子；在B淋巴瘤细胞中，NIP45与NF-ATp和c-maf瞬时共表达可诱导可检测到的内源性IL-4蛋白产生。NIP45的鉴定增进了我们对细胞因子基因激活的理解，细胞因子是免疫反应的关键介质。
