小鼠rad51基因的一个突变会导致早期胚胎致死，而这种致死效应会被p53基因的一个突变所抑制。
A mutation in mouse rad51 results in an early embryonic lethal that is suppressed by a mutation in p53.
作者信息
Lim D S, Hasty P
机构信息
Department of Biochemistry and Molecular Biology, M.D. Anderson Cancer Center, Houston, Texas 77030, USA.
出版信息
Mol Cell Biol. 1996 Dec;16(12):7133-43. doi: 10.1128/MCB.16.12.7133.
Abstract
RecA in Escherichia coli and its homolog, ScRad51 in Saccharomyces cerevisiae, are known to be essential for recombinational repair. The homolog of RecA and ScRad51 in mice, MmRad51, was mutated to determine its function. Mutant embryos arrested early during development. A decrease in cell proliferation, followed by programmed cell death and chromosome loss, was observed. Radiation sensitivity was demonstrated in trophectoderm-derived cells. Interestingly, embryonic development progressed further in a p53 null background; however, fibroblasts derived from double-mutant embryos failed to proliferate in tissue culture.
摘要
已知大肠杆菌中的RecA及其在酿酒酵母中的同源物ScRad51对重组修复至关重要。对小鼠中RecA和ScRad51的同源物MmRad51进行突变以确定其功能。突变胚胎在发育早期停滞。观察到细胞增殖减少，随后是程序性细胞死亡和染色体丢失。在滋养外胚层来源的细胞中表现出辐射敏感性。有趣的是，在p53基因缺失的背景下胚胎发育进一步进行；然而，来自双突变胚胎的成纤维细胞在组织培养中无法增殖。
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本文引用的文献
1
The cellular responses to DNA damage.细胞对DNA损伤的反应。
Trends Cell Biol. 1995 Jan;5(1):32-40. doi: 10.1016/s0962-8924(00)88934-5.
2
3
Chromosomal double-strand break repair in Ku80-deficient cells.Ku80 缺陷细胞中的染色体双链断裂修复
Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8929-33. doi: 10.1073/pnas.93.17.8929.
4
Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates.Ku86基因缺陷型小鼠表现出严重联合免疫缺陷以及V(D)J重组中间体加工缺陷。
Cell. 1996 Aug 9;86(3):379-89. doi: 10.1016/s0092-8674(00)80111-7.
5
Requirement for Ku80 in growth and immunoglobulin V(D)J recombination.生长及免疫球蛋白V(D)J重组过程中对Ku80的需求。
Nature. 1996 Aug 8;382(6591):551-5. doi: 10.1038/382551a0.
6
Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication.酵母中缺乏RAD51时的双链断裂修复：断裂诱导的DNA复制的可能作用。
Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7131-6. doi: 10.1073/pnas.93.14.7131.
7
Targeted disruption of the Rad51 gene leads to lethality in embryonic mice.对Rad51基因进行靶向破坏会导致胚胎期小鼠死亡。
Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6236-40. doi: 10.1073/pnas.93.13.6236.
8
p53: puzzle and paradigm.p53：谜题与范式
Genes Dev. 1996 May 1;10(9):1054-72. doi: 10.1101/gad.10.9.1054.
9
Cell cycle-dependent expression of the mouse Rad51 gene in proliferating cells.小鼠Rad51基因在增殖细胞中的细胞周期依赖性表达。
Mol Gen Genet. 1996 Apr 24;251(1):1-12. doi: 10.1007/BF02174338.
10
Positional cloning of the Werner's syndrome gene.沃纳综合征基因的定位克隆
Science. 1996 Apr 12;272(5259):258-62. doi: 10.1126/science.272.5259.258.
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