Yamamoto A, Taki T, Yagi H, Habu T, Yoshida K, Yoshimura Y, Yamamoto K, Matsushiro A, Nishimune Y, Morita T
Department of Molecular Embryology, Research Institute of Microbial Diseases, Osaka, Japan.
Mol Gen Genet. 1996 Apr 24;251(1):1-12. doi: 10.1007/BF02174338.
The mouse Rad51 gene is a mammalian homologue of the Escherichia coli recA and yeast RAD51 genes, both of which are involved in homologous recombination and DNA repair in mitosis and meiosis. The expression of mouse Rad51 mRNA was examined in synchronized mouse m5S cells. The Rad51 transcript was observed from late G1 phase through to M phase. During the period of late G1-S-G2, the RAD51 proteins were observed exclusively in nuclei. Activation by mitogens of T cell and B cell proliferation in spleen induced the expression of Rad51 mRNA. By immunohistochemical analyses, in mouse RAD51 protein was detected in proliferating cells: spermatogonia in testis, immature T cells in thymus, germinal center cells of the secondary lymphatic nodules of spleen and intestine, follicle cells in ovary and epithelial cells in uterus and intestine. It was also expressed in spermatocytes during early and mid-prophase of meiosis and in resting oocytes before maturation. Thus, mouse Rad51 expression is closely related to the state of cell proliferation and is presumably involved in DNA repair coupled with DNA replication, as well as in meiotic DNA recombination in spermatocytes.
小鼠Rad51基因是大肠杆菌recA基因和酵母RAD51基因的哺乳动物同源物，这两个基因都参与有丝分裂和减数分裂中的同源重组及DNA修复。在同步化的小鼠m5S细胞中检测了小鼠Rad51 mRNA的表达。从G1期晚期到M期均观察到Rad51转录本。在G1-S-G2晚期，RAD51蛋白仅在细胞核中被观察到。脾脏中T细胞和B细胞增殖的有丝分裂原激活诱导了Rad51 mRNA的表达。通过免疫组织化学分析，在增殖细胞中检测到小鼠RAD51蛋白：睾丸中的精原细胞、胸腺中的未成熟T细胞、脾脏和肠道二级淋巴小结的生发中心细胞、卵巢中的卵泡细胞以及子宫和肠道中的上皮细胞。它在减数分裂前期早期和中期的精母细胞以及成熟前的静止卵母细胞中也有表达。因此，小鼠Rad51表达与细胞增殖状态密切相关，推测其参与了与DNA复制相关的DNA修复以及精母细胞中的减数分裂DNA重组。
