Andreani C L, Pierro E, Lazzarin N, Lanzone A, Caruso A, Mancuso S
Istituto di Ginecologia e Ostetricia dell'Università Cattolica del Sacro Cuore, Roma, Italy.
Hum Reprod. 1996 Oct;11(10):2107-13. doi: 10.1093/oxfordjournals.humrep.a019057.
Recent data suggest that follicular fluid may play an important role in the endocrine balance of polycystic ovary syndrome, probably by acting on the theca-granulosa cell relationship. The aim of this study was to evaluate the effect of steroid-free follicular fluid on steroidal response and cell proliferation of human granulosa luteal cells from polycystic (POGC) and normal ovary (NC). Granulosa cells (from both POGC and NC) were cultured for 48 h with or without increasing dilutions of follicular fluid (FF) obtained from polycystic (FFp) and normo-ovulating (FFc) patients. Both follicular fluids were able to elicit aromatase activity as well as progesterone production and thymidine incorporation. POGC, when incubated with FFp, showed a lower increase of aromatase activity and progesterone production with respect to NC. Furthermore, the proliferation rate was increased by incubation with either follicular fluid, but the increase was less with FFp compared to FFc. Aromatase/[3H]thymidine (A/T) and progesterone/ [3H]thymidine (P/T) ratios could be considered to be representative of the contribution of the single cell unit to steroidogenesis. Using high concentrations of either follicular fluids, POGC showed a higher A/T ratio compared with NC. Moreover, the same treatment strongly decreased P/T ration in POGC, while it was ineffective in NC. Our study show that an abnormal interaction between POGC and their own follicular fluid can be implicated in the pathogenesis of the altered steroidal response in these cells, and that in particular it could affect the proliferation rate.
近期数据表明，卵泡液可能在多囊卵巢综合征的内分泌平衡中发挥重要作用，可能是通过作用于卵泡膜细胞-颗粒细胞关系来实现的。本研究的目的是评估无类固醇卵泡液对多囊卵巢（POGC）和正常卵巢（NC）来源的人颗粒黄体细胞甾体反应和细胞增殖的影响。颗粒细胞（来自POGC和NC）在有或没有来自多囊患者（FFp）和正常排卵患者（FFc）的卵泡液（FF）不同稀释度的情况下培养48小时。两种卵泡液均能引发芳香化酶活性以及孕酮生成和胸苷掺入。与NC相比，POGC与FFp孵育时，芳香化酶活性和孕酮生成的增加较低。此外，与任一卵泡液孵育均可增加增殖率，但与FFc相比，FFp孵育时增殖率增加较少。芳香化酶/[3H]胸苷（A/T）和孕酮/[3H]胸苷（P/T）比值可被视为单细胞单位对类固醇生成贡献的代表。使用高浓度的任一卵泡液时，POGC与NC相比显示出更高的A/T比值。此外，相同处理可使POGC中的P/T比值大幅降低，而对NC则无效。我们的研究表明，POGC与其自身卵泡液之间的异常相互作用可能与这些细胞中甾体反应改变的发病机制有关，特别是可能会影响增殖率。
