Navarra P, Andreani C L, Lazzarin N, Pierro E, Mirtella A, Lanzone A, Mancuso S
Institutes of Pharmacology, Catholic University Medical School, Rome, Italy.
Prostaglandins. 1996 Sep;52(3):187-97. doi: 10.1016/s0090-6980(96)00096-2.
This study was conducted to compare the levels of prostaglandin E2 (PGE2) released by cultured granulosa cells collected from normally-ovulating women (normal cells, NC) and those with polycystic ovaries (polycystic ovary granulosa cells, POGC). Granulosa cells were collected from 7 normal women and 7 anovulatory women with polycystic ovaries. Both groups underwent laparoscopic oocyte retrieval for gamete intra-fallopian transfer. Cell cultures were carried out under basal conditions and in the presence of various substances known to influence PGE2 biosynthesis. Prostaglandin E2 concentrations in the incubation media were taken as a marker of cyclo-oxygenase activity. Unexpectedly, POGC appeared to release greater amounts of PGE2 compared to the NC. There was no difference between the levels of PGE2 produced by the two types of cells during the first 3 hours after cell explants, whereas a difference (P < 0.01) was observed after 24 and 48 hours of incubation. Interleukin-1 beta enhanced PGE2 secretion (P < 0.01) in both POGC and NC, while lipopolysaccharide increased prostaglandin release only by the NC cells. Indomethacin inhibited PGE2 production to a greater extent in POGC (from -70 to -90% with respect to basal release, P < 0.01) than NC (approximately -50%, P < 0.01). Blockade by indomethacin and the weak inhibitory effect of the glucocorticoid, dexamethasone (P < 0.05 only in NC, and only at 24 hours), provided pharmacological evidence that PG production by granulosa cells in vitro might depend primarily on constitutive cyclo-oxygenase activity.
本研究旨在比较从正常排卵女性（正常细胞，NC）和多囊卵巢女性（多囊卵巢颗粒细胞，POGC）收集的培养颗粒细胞释放的前列腺素E2（PGE2）水平。从7名正常女性和7名患有多囊卵巢的无排卵女性中收集颗粒细胞。两组均接受腹腔镜取卵以进行配子输卵管内移植。细胞培养在基础条件下以及在存在各种已知影响PGE2生物合成的物质的情况下进行。孵育培养基中的前列腺素E2浓度被用作环氧化酶活性的标志物。出乎意料的是，与NC相比，POGC似乎释放出更多的PGE2。在细胞外植体后的前3小时内，两种类型的细胞产生的PGE2水平没有差异，而在孵育24小时和48小时后观察到差异（P<0.01）。白细胞介素-1β增强了POGC和NC中PGE2的分泌（P<0.01），而脂多糖仅增加了NC细胞的前列腺素释放。吲哚美辛在POGC中比在NC中更能抑制PGE2的产生（相对于基础释放从-70%到-90%，P<0.01）（在NC中约为-50%，P<0.01）。吲哚美辛的阻断作用以及糖皮质激素地塞米松的微弱抑制作用（仅在NC中P<0.05，且仅在24小时时）提供了药理学证据，表明体外颗粒细胞产生PG可能主要取决于组成型环氧化酶活性。
