Sun X F, Carstensen J M, Zhang H, Arbman G, Nordenskjöld B
Department of Oncology, Linköping University, Sweden.
Eur J Cancer. 1996 Oct;32A(11):1963-7. doi: 10.1016/0959-8049(96)00205-5.
The prognostic significance of nuclear and cytoplasmic p53 protein, detected immunocytochemically using CM1 and PAb 1801 antibodies, was evaluated in right-sided and left-sided colorectal adenocarcinomas from 293 patients. CM1 nuclear and cytoplasmic p53 accumulation occurred in 38 and 25% of cases, respectively. PAb 1801 nuclear staining occurred in 18%, with no cytoplasmic staining. CM1 expression either in the nucleus or in the cytoplasm was positively related to PAb 1801 expression (P < 0.001 and P = 0.009, respectively). The incidence of CM1 nuclear and cytoplasmic expression was more frequent in right-sided tumours (P = 0.023 and P = 0.034, respectively), while PAb 1801 nuclear staining was more common in left-sided tumours (P = 0.011). In survival analyses, CM1 nuclear overexpression in the right-sided tumours (P = 0.016) and CM1 cytoplasmic overexpression in left-sided tumours (P = 0.04) were prognostic indicators, independent of Dukes' stage, DNA ploidy, PAb 1801 expression and each other. Further analysis showed that the prognostic value of CM1 nuclear expression was greater in right-sided tumours than in left-sided tumours (P = 0.018). The nuclear and cytoplasmic p53 protein detected with CM1 and PAb 1801 may play different roles in tumour progression and provide prognostic indicators for right- and left-sided colorectal tumours.
使用CM1和PAb 1801抗体通过免疫细胞化学方法检测核及胞质p53蛋白，评估其在293例左右侧结肠腺癌患者中的预后意义。CM1核及胞质p53蛋白积累分别出现在38%和25%的病例中。PAb 1801核染色出现在18%的病例中，无胞质染色。CM1在细胞核或细胞质中的表达与PAb 1801表达呈正相关（分别为P < 0.001和P = 0.009）。CM1核及胞质表达的发生率在右侧肿瘤中更高（分别为P = 0.023和P = 0.034），而PAb 1801核染色在左侧肿瘤中更常见（P = 0.011）。在生存分析中，右侧肿瘤中CM1核过表达（P = 0.016）和左侧肿瘤中CM1胞质过表达（P = 0.04）是预后指标，独立于Dukes分期、DNA倍体、PAb 1801表达且相互独立。进一步分析表明，CM1核表达在右侧肿瘤中的预后价值大于左侧肿瘤（P = 0.018）。用CM1和PAb 1801检测到的核及胞质p53蛋白可能在肿瘤进展中发挥不同作用，并为左右侧结肠肿瘤提供预后指标。
