Greenfield A, Scott D, Pennisi D, Ehrmann I, Ellis P, Cooper L, Simpson E, Koopman P
Centre for Molecular & Cellular Biology, University of Queensland, Brisbane, Australia.
Nat Genet. 1996 Dec;14(4):474-8. doi: 10.1038/ng1296-474.
Rejection of male tissue grafts by genotypically identical female mice has been explained by the existence of a male-specific transplantation antigen, H-Y (ref. 1), but the molecular nature of H-Y antigen has remained obscure. Hya, the murine locus controlling H-Y expression, has been localized to delta Sxrb, a deletion interval of the short arm of the Y chromosome. In mice, H-Y antigen comprises at least four distinct epitopes, each recognized by a specific T lymphocyte clone. It has recently been shown that one of these epitopes, H-YKk, is a peptide encoded by the Y-linked Smcy gene, presented at the cell surface with the H-2Kk major histocompatibility complex (MHC) molecule. However, deletion mapping and the analysis of variable inactivation of H-Y epitopes has suggested that the Hya locus may be genetically complex. Here we describe a novel mouse Y chromosome gene which we call Uty (ubiquitously transcribed tetratricopeptide repeat gene on the Y chromosome). We identify the peptide WMHHNMDLI derived from the UTY protein as an H-Y epitope, H-YDb. Our data formally demonstrate that H-Y antigen is the product of more than one gene on the Y chromosome.
基因相同的雌性小鼠对雄性组织移植物的排斥反应已通过存在一种雄性特异性移植抗原H-Y（参考文献1）来解释，但H-Y抗原的分子本质仍不清楚。Hya是控制H-Y表达的小鼠基因座，已被定位到Y染色体短臂的缺失区间δSxrb。在小鼠中，H-Y抗原至少包含四个不同的表位，每个表位都由一个特定的T淋巴细胞克隆识别。最近已经表明，这些表位之一H-YKk是由Y连锁的Smcy基因编码的一种肽，与H-2Kk主要组织相容性复合体（MHC）分子一起呈递在细胞表面。然而，缺失图谱分析和H-Y表位可变失活分析表明，Hya基因座可能在遗传上是复杂的。在这里，我们描述了一个新的小鼠Y染色体基因，我们称之为Uty（Y染色体上普遍转录的四肽重复基因）。我们将源自UTY蛋白的肽WMHHNMDLI鉴定为一种H-Y表位H-YDb。我们的数据正式证明H-Y抗原是Y染色体上多个基因的产物。
