Birnbaum Y, Hale S L, Kloner R A
Heart Institute, Good Samaritan Hospital, Los Angeles, CA 90017, USA.
Cardiovasc Res. 1996 Nov;32(5):861-8.
Coenzyme Q10 has been found to enhance recovery of function after reperfusion in numerous experimental acute ischemia-reperfusion models. We assessed whether coenzyme Q10, administered intravenously either during or 1 h before ischemia, can limit infarct size in the rabbit.
Anesthetized open-chest rabbits were subjected to 30 min of coronary artery occlusion and 4 h of reperfusion. In Protocol 1, 12 min after beginning of ischemia rabbits were randomized to intravenous infusion of 30 mg coenzyme Q10 (Eisai Co., Japan) (n = 10) or vehicle (n = 10). In Protocol 2, rabbits were randomized to 30 mg coenzyme Q10 (n = 6) or vehicle (n = 6) treatment 60 min before ischemia. Ischemic zone at risk (IZ) was assessed by blue dye and necrotic zone (NZ) by tetrazolium staining.
In both protocols, coenzyme Q10 did not alter heart rate, mean blood pressure, or regional myocardial blood flows in either the ischemic or non-ischemic zones during ischemia or reperfusion. No difference was found in IZ (as fraction of LV weight) (Protocol 1: 0.24 +/- 0.02 vs. 0.25 +/- 0.02; Protocol 2: 0.28 +/- 0.02 vs. 0.28 +/- 0.03, in the control vs. coenzyme Q10 groups, respectively). The NZ/IZ ratio was comparable between the groups in both protocols (Protocol 1: 0.22 +/- 0.04 vs. 0.26 +/- 0.04; Protocol 2: 0.21 +/- 0.06 vs. 0.30 +/- 0.06, in the control vs. coenzyme Q10 groups, respectively).
Coenzyme Q10, administered acutely either during or 60 min before myocardial ischemia, does not attenuate infarct size in the rabbit.
在众多实验性急性缺血再灌注模型中，已发现辅酶Q10可增强再灌注后功能的恢复。我们评估了在缺血期间或缺血前1小时静脉给予辅酶Q10是否能限制家兔的梗死面积。
对麻醉开胸的家兔进行30分钟冠状动脉闭塞和4小时再灌注。在方案1中，缺血开始12分钟后，将家兔随机分为静脉输注30毫克辅酶Q10（日本卫材株式会社）（n = 10）或赋形剂（n = 10）组。在方案2中，家兔在缺血前60分钟随机分为30毫克辅酶Q10（n = 6）或赋形剂（n = 6）治疗组。通过蓝色染料评估危险缺血区（IZ），通过四氮唑染色评估坏死区（NZ）。
在两个方案中，辅酶Q10在缺血或再灌注期间均未改变家兔的心率、平均血压或缺血区和非缺血区的局部心肌血流量。在IZ（占左心室重量的比例）方面未发现差异（方案1：对照组与辅酶Q10组分别为0.24±0.02对0.25±0.02；方案2：分别为0.28±0.02对0.28±0.03）。在两个方案中，各组之间的NZ/IZ比值相当（方案1：对照组与辅酶Q10组分别为0.22±0.04对0.26±0.04；方案2：分别为0.21±0.06对0.30±0.06）。
在心肌缺血期间或缺血前60分钟急性给予辅酶Q10，并不会减小家兔的梗死面积。
