Medalie D A, Tompkins R G, Morgan J R
Surgical Services, Massachusetts General Hospital, Boston, Massachusetts, USA.
ASAIO J. 1996 Sep-Oct;42(5):M455-62. doi: 10.1097/00002480-199609000-00030.
This study evaluated the structure and function of a dermal substitute composed of de-epidermalized, acellular human dermis, and tested its performance as part of a composite skin graft with cultured human keratinocytes. The acellular dermis retained much of the complex structure of native dermis, as demonstrated by immunostaining for collagen Types IV and VII. Collagen Types IV and VII were found on the papillary surface, and collagen Type IV was found at sites throughout the acellular dermis. To further demonstrate the functionality of the acellular dermis material, we seeded the papillary surface with cultured keratinocytes. In response to the architecture of the dermal papillae, the keratinocytes formed a three-dimensional epidermal structure that was several cell layers thick, and recreated the original skin rete ridges at the interface with the dermis. When these composite grafts were transplanted to athymic mice, host fibrovascular cells repopulated the acellular dermis. Some vessels grew into the acellular dermis along the original pathways of the human blood vessels, as demonstrated by co-localization of human and mouse collagen Type IV. The skin that developed from these grafts repigmented completely via passenger melanocytes from the keratinocyte cultures, was durable, and remained stable for more than 5 months.
本研究评估了一种由去表皮、无细胞人真皮组成的真皮替代物的结构和功能，并测试了其作为与培养的人角质形成细胞组成的复合皮肤移植物一部分的性能。如通过对IV型和VII型胶原进行免疫染色所示，无细胞真皮保留了天然真皮的许多复杂结构。在乳头表面发现了IV型和VII型胶原，并且在整个无细胞真皮的部位都发现了IV型胶原。为了进一步证明无细胞真皮材料的功能，我们在乳头表面接种了培养的角质形成细胞。响应于真皮乳头的结构，角质形成细胞形成了几层细胞厚的三维表皮结构，并在与真皮的界面处重新形成了原始的皮肤 rete 嵴。当将这些复合移植物移植到无胸腺小鼠时，宿主纤维血管细胞重新填充了无细胞真皮。如人IV型胶原和小鼠IV型胶原的共定位所示，一些血管沿着人血管的原始路径长入无细胞真皮。由这些移植物形成的皮肤通过角质形成细胞培养物中的过客黑素细胞完全重新色素沉着，耐用且在5个月以上保持稳定。
