Medalie D A, Eming S A, Collins M E, Tompkins R G, Yarmush M L, Morgan J R
Surgical Services, Massachusetts General Hospital, and the Shriners Burns Institute, Boston 02114, USA.
Transplantation. 1997 Aug 15;64(3):454-65. doi: 10.1097/00007890-199708150-00015.
This study evaluated the in vitro and in vivo function of composite skin equivalents based on two different dermal analogs. Keratinocytes derived from the same dark-skinned neonatal foreskins were seeded onto both acellular human dermis and fibroblast-contracted collagen gels. Each type of composite graft readily formed an epithelium in vitro. However, the undulating surface of the acellular dermis acted as a template and organized the seeded keratinocytes into a rete ridge-like pattern, whereas the smooth surface of the fibroblast-contracted collagen gels generated an epithelium with a linear basal layer. Moreover, when acellular dermis was used, the composite grafts demonstrated enhanced melanocyte proliferation. When transplanted to athymic mice, both composite grafts formed a fully differentiated human epidermis, but repigmentation of the grafts when acellular dermis was used was more extensive and only the epidermis on the fibroblast-contracted collagen gels showed signs of hyperproliferation at 6 weeks after grafting. These results demonstrate that the type of dermal analog incorporated into a composite skin graft can influence the subsequent functionality of the skin substitute.
本研究评估了基于两种不同真皮类似物的复合皮肤替代物的体外和体内功能。将源自同一深色皮肤新生儿包皮的角质形成细胞接种到脱细胞人真皮和成纤维细胞收缩的胶原凝胶上。每种类型的复合移植物在体外均易于形成上皮。然而，脱细胞真皮的起伏表面充当模板，将接种的角质形成细胞组织成 rete 嵴样模式，而成纤维细胞收缩的胶原凝胶的光滑表面产生具有线性基底层的上皮。此外，当使用脱细胞真皮时，复合移植物显示出黑素细胞增殖增强。当移植到无胸腺小鼠时，两种复合移植物均形成完全分化的人表皮，但使用脱细胞真皮时移植物的色素再沉着更广泛，并且仅成纤维细胞收缩的胶原凝胶上的表皮在移植后 6 周显示出增殖过度的迹象。这些结果表明，纳入复合皮肤移植物中的真皮类似物类型可影响皮肤替代物的后续功能。
