Rafie-Kolpin M, Essenberg R C, Wyckoff J H
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078, USA.
Infect Immun. 1996 Dec;64(12):5274-83. doi: 10.1128/iai.64.12.5274-5283.1996.
Brucella abortus is a facultative intracellular pathogen of cattle and humans that is capable of survival inside macrophages. In order to understand how B. abortus copes with the conditions during intracellular growth in macrophages, the protein synthesis pattern of the bacteria grown inside bovine macrophages has been compared with that of bacteria grown in the cell culture medium by two-dimensional polyacrylamide gel electrophoresis. Approximately 24 new proteins that are not detected in the bacteria grown in the cell culture medium have been induced during intracellular growth in macrophages. In contrast, approximately 50 proteins that were expressed during growth in cell culture medium were completely repressed during intracellular growth. The level of expression of 19 proteins increases while that of 54 proteins decreases during intracellular growth. To understand these results, the protein synthesis pattern of B. abortus during intracellular growth was compared with those during other stress conditions. Under each stress condition studied, several new proteins were induced that were not present during regular growth conditions. Comparison of the protein synthesis pattern of B. abortus during intracellular growth with those obtained under various stress conditions has indicated that the response to intracellular growth was not just a simple sum of stress conditions studied so far.
流产布鲁氏菌是一种兼性细胞内病原体，可感染牛和人类，能够在巨噬细胞内存活。为了了解流产布鲁氏菌如何应对在巨噬细胞内生长期间的环境条件，通过二维聚丙烯酰胺凝胶电泳，对在牛巨噬细胞内生长的细菌的蛋白质合成模式与在细胞培养基中生长的细菌的蛋白质合成模式进行了比较。在巨噬细胞内生长期间，诱导产生了约24种在细胞培养基中生长的细菌中未检测到的新蛋白质。相反，在细胞培养基中生长时表达的约50种蛋白质在细胞内生长期间完全受到抑制。在细胞内生长期间，19种蛋白质的表达水平增加，而54种蛋白质的表达水平下降。为了理解这些结果，将流产布鲁氏菌在细胞内生长期间的蛋白质合成模式与在其他应激条件下的蛋白质合成模式进行了比较。在所研究的每种应激条件下，都诱导产生了几种在正常生长条件下不存在的新蛋白质。将流产布鲁氏菌在细胞内生长期间的蛋白质合成模式与在各种应激条件下获得的蛋白质合成模式进行比较表明，对细胞内生长的反应不仅仅是迄今为止所研究的应激条件的简单总和。
