抗内化素富含亮氨酸重复序列区域的抗体可阻断单核细胞增生李斯特菌进入表达E-钙黏蛋白的细胞。
Antibodies to the leucine-rich repeat region of internalin block entry of Listeria monocytogenes into cells expressing E-cadherin.
作者信息
Mengaud J, Lecuit M, Lebrun M, Nato F, Mazie J C, Cossart P
机构信息
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France.
出版信息
Infect Immun. 1996 Dec;64(12):5430-3. doi: 10.1128/iai.64.12.5430-5433.1996.
Abstract
Internalin, a surface protein essential for entry of Listeria monocytogenes EGD into epithelial cells, was used as an antigen to raise nine monoclonal antibodies. These monoclonal antibodies recognized seven distinct epitopes which were located in three different regions of the protein. Three of them inhibited internalin-mediated entry and recognized the amino-terminal leucine-rich repeat region of the protein, suggesting that this region is essential for entry.
摘要
内化素是单核细胞增生李斯特菌EGD株进入上皮细胞所必需的一种表面蛋白，被用作抗原以产生9种单克隆抗体。这些单克隆抗体识别7个不同的表位，它们位于该蛋白的3个不同区域。其中3种抗体抑制内化素介导的进入，并识别该蛋白的氨基末端富含亮氨酸的重复区域，这表明该区域对进入至关重要。
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本文引用的文献
1
Cellular resistance to infection.细胞抗感染能力。
J Exp Med. 1962 Sep 1;116(3):381-406. doi: 10.1084/jem.116.3.381.
2
Internalin must be on the bacterial surface to mediate entry of Listeria monocytogenes into epithelial cells.内化素必须位于细菌表面，以介导单核细胞增生李斯特菌进入上皮细胞。
Mol Microbiol. 1996 Aug;21(3):579-92. doi: 10.1111/j.1365-2958.1996.tb02566.x.
3
Molecular and genetic determinants involved in invasion of mammalian cells by Listeria monocytogenes.单核细胞增生李斯特菌侵袭哺乳动物细胞所涉及的分子和遗传决定因素。
Curr Top Microbiol Immunol. 1996;209:61-77. doi: 10.1007/978-3-642-85216-9_4.
4
Uptake of enteropathogenic Yersinia by mammalian cells.哺乳动物细胞对肠道致病性耶尔森氏菌的摄取。
Curr Top Microbiol Immunol. 1996;209:1-24. doi: 10.1007/978-3-642-85216-9_1.
5
E-cadherin is the receptor for internalin, a surface protein required for entry of L. monocytogenes into epithelial cells.E-钙黏蛋白是内化素的受体，内化素是单核细胞增生李斯特菌进入上皮细胞所必需的一种表面蛋白。
Cell. 1996 Mar 22;84(6):923-32. doi: 10.1016/s0092-8674(00)81070-3.
6
Adhesion between epithelial cells and T lymphocytes mediated by E-cadherin and the alpha E beta 7 integrin.上皮细胞与T淋巴细胞之间由E-钙黏蛋白和αEβ7整合素介导的黏附。
Nature. 1994 Nov 10;372(6502):190-3. doi: 10.1038/372190a0.
7
Common features of gram-positive bacterial proteins involved in cell recognition.参与细胞识别的革兰氏阳性菌蛋白质的共同特征。
Mol Microbiol. 1993 Sep;9(5):1119-21. doi: 10.1111/j.1365-2958.1993.tb01241.x.
8
Molecular and genetic determinants of the Listeria monocytogenes infectious process.单核细胞增生李斯特菌感染过程的分子和遗传决定因素。
Curr Top Microbiol Immunol. 1994;192:187-216. doi: 10.1007/978-3-642-78624-2_9.
9
Recognition of E-cadherin on epithelial cells by the mucosal T cell integrin alpha M290 beta 7 (alpha E beta 7).黏膜T细胞整合素αM290β7（αEβ7）对上皮细胞上E-钙黏蛋白的识别。
Eur J Immunol. 1995 Mar;25(3):852-6. doi: 10.1002/eji.1830250333.
10
Entry of Listeria monocytogenes into hepatocytes requires expression of inIB, a surface protein of the internalin multigene family.单核细胞增生李斯特菌进入肝细胞需要表达内化素B（inIB），它是内化素多基因家族的一种表面蛋白。
Mol Microbiol. 1995 Apr;16(2):251-61. doi: 10.1111/j.1365-2958.1995.tb02297.x.
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