住院治疗毛细支气管炎的儿童中肺炎衣原体和沙眼衣原体的巢式聚合酶链反应检测
The nPCR detection of Chlamydia pneumoniae and Chlamydia trachomatis in children hospitalized for bronchiolitis.
作者信息
Khan M A, Potter C W
机构信息
Department of Medical Microbiology, Medical School, University of Sheffield, U.K.
出版信息
J Infect. 1996 Nov;33(3):173-5. doi: 10.1016/s0163-4453(96)92177-5.
Nasopharyngeal aspirates from children admitted with the diagnosis of bronchiolitis, were screened for Chlamydia pneumoniae, and C. trachomatis. The nested PCR was found to be more sensitive that amplified the DNA extracts of up to two elementary bodies of the test strains. Using this technique, C. pneumoniae was detected in two (1.3%), and C. trachomatis in 26 17.1%) of the 152 samples tested. This study indicated C. pneumoniae infections to be unusual in children with bronchiolitis.
对因毛细支气管炎入院的儿童的鼻咽抽吸物进行了肺炎衣原体和沙眼衣原体筛查。发现巢式聚合酶链反应（nested PCR）更敏感，它能扩增测试菌株多达两个原体的DNA提取物。使用该技术，在152份测试样本中，检测到2份（1.3%）肺炎衣原体感染，26份（17.1%）沙眼衣原体感染。这项研究表明，肺炎衣原体感染在毛细支气管炎患儿中并不常见。
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