Kowalczyk D, Skorupski W, Kwias Z, Nowak J
Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.
Immunol Lett. 1996 Oct;53(1):15-8. doi: 10.1016/0165-2478(96)02605-3.
Gamma/delta (gamma delta) T lymphocytes have been postulated to play a role in a surveillance mechanism that eliminates transformed or otherwise damaged cells. In this study, we examined by flow cytometry the frequency and phenotype of gamma delta T cells in the tumour infiltrating lymphocytes (TIL) and peripheral blood (PBL) from renal cell carcinoma patients. The TCR gamma delta + cells comprised an average of 3.8% of the CD3+ TIL and 5.2% of circulating T cells. Analysis of surface immunophenotype revealed that activation markers of T lymphocytes: CD25 and HLA DR were highly expressed on the tumour infiltrating gamma delta + T lymphocytes (median 27.6% for CD25 and 52.0% for HLA DR). More importantly, percentage of activated gamma delta T cells was found to be much higher than compared to all activated CD3+ cells. Furthermore, an unusually high proportion of gamma delta positive TILs express CD4 or CD8 molecules (17.2 and 36.8%, respectively), indicating that they might recognise antigen presented within MHC II or I context. These results suggest that gamma delta T lymphocytes may play a certain role in immune response against tumour cells.
γ/δ（伽马/德尔塔）T淋巴细胞被推测在一种清除转化细胞或其他受损细胞的监视机制中发挥作用。在本研究中，我们通过流式细胞术检测了肾细胞癌患者肿瘤浸润淋巴细胞（TIL）和外周血（PBL）中γδ T细胞的频率和表型。TCRγδ +细胞平均占CD3 + TIL的3.8%和循环T细胞的5.2%。表面免疫表型分析显示，T淋巴细胞的激活标志物：CD25和HLA DR在肿瘤浸润的γδ + T淋巴细胞上高表达（CD25中位数为27.6%，HLA DR为52.0%）。更重要的是，发现活化的γδ T细胞百分比比所有活化的CD3 +细胞高得多。此外，γδ阳性TIL中异常高比例表达CD4或CD8分子（分别为17.2%和36.8%），表明它们可能识别在MHC II或I背景下呈递的抗原。这些结果表明，γδ T淋巴细胞可能在针对肿瘤细胞的免疫反应中发挥一定作用。
