Alam S M, Clark J S, Leech V, Whitford P, George W D, Campbell A M
Department of Biochemistry, University of Glasgow, U.K.
Immunol Lett. 1992 Feb 15;31(3):279-83. doi: 10.1016/0165-2478(92)90127-a.
The quantitative distribution and phenotype of gamma/delta lymphocytes in the peripheral blood (PBL), tumour draining lymph node (LNL) and tumour infiltrating lymphocytes (TIL) from breast carcinoma patients were determined by one- and two-colour flow cytometry. The TCR-gamma/delta + cells generally expressed the T cell lineage antigen CD3. The proportions of such cells were variable but generally small from all the three sources. Phenotypic analysis revealed that the CD8 marker was consistently and predominantly observed on gamma/delta + CD3+ cells in the tumour infiltrate, whereas CD4 expression, while generally low, was noted on a significant percentage (median 10%) of LNL gamma/delta + lymphocytes. In both PBL and LNL the predominant gamma/delta cell population was CD4-8-.
采用单染色和双染色流式细胞术测定乳腺癌患者外周血（PBL）、肿瘤引流淋巴结（LNL）和肿瘤浸润淋巴细胞（TIL）中γ/δ淋巴细胞的定量分布和表型。TCR-γ/δ +细胞通常表达T细胞系抗原CD3。这些细胞的比例各不相同，但在所有这三种来源中通常都很少。表型分析显示，在肿瘤浸润物中的γ/δ + CD3+细胞上始终且主要观察到CD8标志物，而CD4表达虽然通常较低，但在相当比例（中位数10%）的LNL γ/δ +淋巴细胞上有表达。在PBL和LNL中，主要的γ/δ细胞群都是CD4-8-。
