Matata B M, Yin H Q, Courtney J M, Gaylor J D, Lamba N M, Lowe G D, Suzuki K, Kimura H, Izumi K, Klinkmann H
Bioengineering Unit, University of Strathclyde, Glasgow, UK.
Int J Artif Organs. 1996 Oct;19(10):582-9.
A procedure has been established for the in vitro assessment of hollow fibre haemodialysis membranes. A 30 ml syringe containing 20 ml of fresh non-anticoagulated blood was mounted onto a non-pulsatile syringe pump and blood was perfused through minimodules constructed from 80 fibres retrieved from Cuprophan (Baxter ST15), cellulose acetate (M57-12, JMS Co Ltd, Hiroshima, Japan), and AN69HF (Filtral 20, Hospal, France) dialysers. Samples were collected before perfusion, 3, 6, 9 and 12 minutes. The modules were clamped vertically to minimise the effect of red cell pooling and the dialysate compartment was filled with 0.9% saline to minimise ultrafiltration. After sample processing, complement C3a, thrombin-antithrombin III complexes, prothrombin F1 + 2, and factor XII-like activity were evaluated. The results indicated that the system could discriminate between the membranes evaluated and therefore was a relevant procedure for the assessment of hollow fibre haemodialysis membranes.
已建立一种用于中空纤维血液透析膜体外评估的程序。将一支装有20 ml新鲜未抗凝血液的30 ml注射器安装到无脉动的注射泵上，血液通过由从铜仿膜（百特ST15）、醋酸纤维素膜（M57 - 12，日本广岛JMS有限公司）和AN69HF膜（费特拉尔20，法国霍斯帕尔公司）透析器中取出的80根纤维构建的微型组件进行灌注。在灌注前、3分钟、6分钟、9分钟和12分钟时采集样本。将微型组件垂直夹紧以尽量减少红细胞聚集的影响，透析液腔填充0.9%生理盐水以尽量减少超滤。样本处理后，评估补体C3a、凝血酶 - 抗凝血酶III复合物、凝血酶原F1 + 2和因子XII样活性。结果表明该系统能够区分所评估的膜，因此是评估中空纤维血液透析膜的一种相关程序。
