Packard M G, Teather L A, Bazan N G
Department of Psychology, University of New Orleans, Louisiana 70148, USA.
Neurobiol Learn Mem. 1996 Sep;66(2):176-82. doi: 10.1006/nlme.1996.0058.
The present experiments examined the effects of posttraining intra-dorsal striatal (i.e., caudate-putamen) injections of the phospholipid methylcarbamyl platelet-activating factor (mc-PAF) and the platelet-activating factor (PAF) antagonist BN 52021 on memory using a striatal-dependent cued water maze task. Male Long-Evans rats received an eight-trial training session in which a visibly cued escape platform was located in a different quadrant of the maze on each trial, followed by an intrastriatal injection of mc-PAF (0.5, 1.0, or 2.0 micrograms/0.5 microliter), BN 52021 (0.25, 0.5, or 1.0 microgram/0.5 microliter), or vehicle (DMSO; 0.5 microliter). On a retention test 24 h later, the escape latencies of rats given mc-PAF (1.0, 2.0 micrograms) wee significantly lower than those of vehicle-injected controls, indicating a memory enhancing effect of mc-PAF. The retention test escape latencies of rats given BN 52021 (0.5, 1.0 microgram) were significantly higher than those of vehicle-injected controls, indicating a memory impairing effect of BN 52021. Injections of mc-PAF or BN 52021 did not affect retention when administered 2-h posttraining, indicating a time-dependent effect of the drugs on memory storage. The findings indicate a role for endogenous PAF function in striatal-dependent memory processes.
本实验采用依赖纹状体的线索水迷宫任务，研究了训练后向背侧纹状体（即尾状核-壳核）注射磷脂甲基氨基甲酰血小板活化因子（mc-PAF）和血小板活化因子（PAF）拮抗剂BN 52021对记忆的影响。雄性Long-Evans大鼠接受了八次训练，每次训练中，一个明显有线索提示的逃生平台位于迷宫的不同象限，随后进行纹状体内注射mc-PAF（0.5、1.0或2.0微克/0.5微升）、BN 52021（0.25、0.5或1.0微克/0.5微升）或溶剂（二甲亚砜；0.5微升）。在24小时后的记忆测试中，注射mc-PAF（1.0、2.0微克）的大鼠的逃生潜伏期显著低于注射溶剂的对照组，表明mc-PAF具有记忆增强作用。注射BN 52021（0.5、1.0微克）的大鼠在记忆测试中的逃生潜伏期显著高于注射溶剂的对照组，表明BN 52021具有记忆损害作用。在训练后2小时注射mc-PAF或BN 52021对记忆保持没有影响，表明药物对记忆存储具有时间依赖性效应。这些发现表明内源性PAF功能在依赖纹状体的记忆过程中发挥作用。
