Sgoifo A, Stilli D, Medici D, Gallo P, Aimi B, Musso E
Dipartimento di Biologia Evolutiva e Funzionale, Università di Parma, Italy.
Physiol Behav. 1996 Dec;60(6):1397-401. doi: 10.1016/s0031-9384(96)00228-4.
We describe a surgical procedure for optimizing the location of telemetry ECG leads in rats. The new location was aimed at obtaining an accurate representation of ECG features throughout the cardiac cycle by limiting the voltage instability usually observed during intense somatomotor activity and improving the signal-to-noise ratio. The two electrodes (wire loops) were fixed on the dorsal surface of the xiphoid process and in the anterior mediastinum close to the right atrium. The implantation procedure was fast, little invasive, and allowed animals to completely recover from intervention. The performance of the "improved" location (IL, n = 10) with respect to two subcutaneous (SC) positionings ("conventional positioning" CSP, n = 5; "updated location," USL, n = 5) was evaluated by comparing ECGs obtained in baseline, stress and recovery conditions and during different behavioral activities (immobility and grooming). The resident-intruder test (emotional/physical challenge) was chosen as experimental stress paradigm. The noise level of ECGs obtained from IL rats was lower than in CSP and USL animals, in all recording conditions. Percentages of correctly recognized beats (CRBs) over the total number of beats (TBs) were significantly higher in IL rats than in CSP and USL animals, both in baseline conditions (99% vs. 11% and 40%) and situations involving high somatomotor activity (stress: 97%, 5% and 16% recovery; 97%, 7% and 15%) (p < 0.01). The performance of IL as compared to CSP and USL was also better when percentages during grooming and immobility were considered (grooming: 93% vs. 4% and 23%: immobility: 97%, 6%, and 33%; p < 0.01).
我们描述了一种用于优化大鼠遥测心电图导联位置的外科手术方法。新位置旨在通过限制在剧烈躯体运动活动期间通常观察到的电压不稳定性并提高信噪比，来准确呈现整个心动周期的心电图特征。两个电极（线环）固定在剑突的背侧表面以及靠近右心房的前纵隔中。植入过程快速、微创，且能使动物从干预中完全恢复。通过比较在基线、应激和恢复条件下以及不同行为活动（静止和梳理毛发）期间获得的心电图，评估了“改进”位置（IL，n = 10）相对于两种皮下（SC）定位（“传统定位”CSP，n = 5；“更新位置”，USL，n = 5）的性能。选择定居者 - 入侵者测试（情绪/身体挑战）作为实验应激范式。在所有记录条件下，从IL大鼠获得的心电图噪声水平均低于CSP和USL动物。在基线条件下（99%对11%和40%）以及涉及高躯体运动活动的情况下（应激：97%、5%和16%；恢复：97%、7%和15%），IL大鼠正确识别搏动（CRB）占总搏动（TB）的百分比显著高于CSP和USL动物（p < 0.01）。当考虑梳理毛发和静止期间的百分比时，IL与CSP和USL相比表现也更好（梳理毛发：93%对4%和23%；静止：97%、6%和33%；p < 0.01）。
