Tabrizi S N, Chen S, Borg A J, Lees M I, Fairley C K, Jackson H D, Gust C H, Migliorini G, Garland S M
Department of Microbiology, Royal Women's Hospital, Carlton, Victoria, Australia.
Sex Transm Dis. 1996 Nov-Dec;23(6):494-7. doi: 10.1097/00007435-199611000-00010.
Diagnosis of genital Chlamydia trachomatis infection in women traditionally requires a speculum examination to collect endocervical cells, followed by cell culture. This method is time consuming, requires stringent transport conditions, and is technically demanding.
To compare tampons as a patient-administered collection method followed by detection with polymerase chain reaction (PCR) with the traditional endocervical swab culture followed by cell culture detection.
At the emergency department of a hospital for obstetrics and gynecology, 1,000 consecutive women with symptoms suggestive of infection with C. trachomatis were tested for C. trachomatis infection by PCR on both tampon (PCR-T) and swab (PCR-S) specimen and by culture of the swab specimen.
Seventeen PCR-T and 16 PCR-S specimens were positive; 16 endocervical specimens were positive by culture, and 14 of the endocervical samples were positive by the three methods. Sixty-one PCR-S samples were inadequate as shown by the lack of amplification of the beta-globin gene segment, indicating poor collection of specimens by endocervical swab for chlamydial testing.
Tampon specimens collected for PCR detection provided an easy and sensitive method of detection of C. trachomatis and overcame the obstacle of endocervical sampling and subsequent stringent transport requirements of culture.
传统上，女性生殖道沙眼衣原体感染的诊断需要进行窥器检查以采集宫颈管细胞，随后进行细胞培养。这种方法耗时、需要严格的运输条件且技术要求高。
比较将棉塞作为患者自行采集方法，随后用聚合酶链反应（PCR）检测，与传统的宫颈拭子培养随后进行细胞培养检测的方法。
在一家妇产科医院的急诊科，对1000名连续出现沙眼衣原体感染症状的女性，通过对棉塞（PCR-T）和拭子（PCR-S）标本进行PCR检测以及对拭子标本进行培养，来检测她们是否感染沙眼衣原体。
17份PCR-T标本和16份PCR-S标本呈阳性；16份宫颈管标本培养呈阳性，其中14份宫颈管样本三种方法检测均呈阳性。61份PCR-S样本因β-珠蛋白基因片段未扩增而不合格，表明宫颈拭子采集衣原体检测标本的效果不佳。
采集棉塞标本用于PCR检测提供了一种简便且灵敏的沙眼衣原体检测方法，克服了宫颈采样及后续培养严格运输要求的障碍。
