Jayakumar R, Thirumurugan G, Nachimuthu K, Padmanaban V D
Department of Animal Biotechnology, Madras Veterinary College, India.
Zentralbl Bakteriol. 1996 Sep;285(1):82-5. doi: 10.1016/s0934-8840(96)80025-9.
An avidin-biotin dot ELISA test was standardized to detect rabies viral antigens from the brain of rabies-suspected animals. This test was compared with the direct fluorescent antibody test (FAT). The advantages of the avidin-biotin dot ELISA are discussed. The incorporation of avidin-biotin into a conventional ELISA is a step forward in improving the available rabies antigen detection procedures as this technique is more sensitive, highly specific and exploits the great affinity of avidin for biotin. No significant difference was observed between FAT and avidin-biotin dot ELISA.
一种抗生物素蛋白-生物素斑点酶联免疫吸附测定法（avidin-biotin dot ELISA test）被标准化，用于检测疑似感染狂犬病动物脑组织中的狂犬病病毒抗原。该检测方法与直接荧光抗体检测法（FAT）进行了比较。文中讨论了抗生物素蛋白-生物素斑点酶联免疫吸附测定法的优点。将抗生物素蛋白-生物素纳入传统酶联免疫吸附测定法是改进现有狂犬病抗原检测程序的一大进步，因为该技术更灵敏、特异性高，且利用了抗生物素蛋白对生物素的高度亲和力。直接荧光抗体检测法和抗生物素蛋白-生物素斑点酶联免疫吸附测定法之间未观察到显著差异。
