Jayakumar R, Nachimuthu K, Padmanaban V D
Department of Animal Biotechnology, Madras Veterinary College, India.
Comp Immunol Microbiol Infect Dis. 1995 Sep;18(4):269-73. doi: 10.1016/0147-9571(95)00007-u.
A modified enzyme linked immunosorbent assay (Dot ELISA) is described for visual detection of rabies antigen in animals. The test materials were dotted onto the nitrocellulose paper and allowed to react with rabies antiserum. The bound antigen--anti-body were reacted with a peroxidase conjugated antirabbit immunoglobulin. Positive reactions were easily visualized as brown dots after enzyme degradation of the substrate. A total of 400 specimens from various geographical locations were tested with the dot ELISA technique, and also with the fluorescent antibody test (FAT), which was used as a reference method. The concordance between the two tests was 95.25%. The dot ELISA may have potential applications as a rapid, simple and economical field test in the diagnosis of rabies.
本文描述了一种改良的酶联免疫吸附测定法（斑点酶联免疫吸附测定法），用于肉眼检测动物体内的狂犬病抗原。将测试材料点样于硝酸纤维素纸上，使其与狂犬病抗血清反应。结合的抗原-抗体再与过氧化物酶标记的抗兔免疫球蛋白反应。酶降解底物后，阳性反应很容易显现为棕色斑点。共使用斑点酶联免疫吸附测定法检测了来自不同地理位置的400份标本，同时也使用荧光抗体试验（FAT）作为参考方法进行检测。两种检测方法的一致性为95.25%。斑点酶联免疫吸附测定法作为一种快速、简单且经济的现场检测方法，在狂犬病诊断中可能具有潜在的应用价值。
