Bridges D A, Roth D M, Cleveland C M, Moran J W, Coleman M R
Division of Eli Lilly and Co., Greenfield, IN 46140-0708, USA.
J AOAC Int. 1996 Nov-Dec;79(6):1255-9.
An existing liquid chromatographic method using postcolumn derivatization has been used extensively to quantitate monensin in animal feeds. Because of the relatively high moisture content of many cattle feed rations, some modifications were made to this method. Several sample-processing steps were evaluated to determine optimum sample-processing procedure. The sample weight/sample diluent ratio was modified, and method linearity was validated for the lower monensin concentrations anticipated in high-moisture cattle rations. The accuracy and precision of data generated at these lower concentrations were also determined. Because of the high moisture content of these rations, data analysis for this method required correction of feed potency for loss on drying. With these modifications, monensin can be accurately determined in high-moisture cattle rations.
一种现有的采用柱后衍生化的液相色谱法已被广泛用于定量分析动物饲料中的莫能菌素。由于许多牛饲料配给的水分含量相对较高，对该方法进行了一些改进。对几个样品处理步骤进行了评估，以确定最佳的样品处理程序。修改了样品重量/样品稀释剂比例，并针对高水分牛饲料配给中预期的较低莫能菌素浓度验证了方法的线性。还确定了在这些较低浓度下生成的数据的准确性和精密度。由于这些饲料配给的水分含量高，该方法的数据分析需要对饲料效力进行干燥失重校正。通过这些改进，可以准确测定高水分牛饲料配给中的莫能菌素。
