Moran J W, Rodewald J M, Donoho A L, Coleman M R
Lilly Research Laboratories, Eli Lilly and Co., Greenfield, IN 46140-0708.
J AOAC Int. 1994 Jul-Aug;77(4):885-90.
A method is described for the detection and quantitation of monensin in chicken tissues by liquid chromatography with postcolumn derivatization with vanillin. Monensin is extracted from the tissues by homogenization with methanol-water and is isolated and concentrated by liquid-liquid partition and sorbent extraction with silica gel. Monensin is mixed postcolumn with vanillin under acidic conditions and heated, and the resulting products are measured by a variable-wavelength detector operating at 520 nm. The method has a limit of quantitation of 0.025 microgram/g and is validated for use in the analyses of chicken muscle, liver, and skin (with adhering fat tissues) for monensin. Standard recoveries from the 3 tissue types tested at 3 levels ranged from 82 to 96%. The method represents an improvement in specificity, accuracy, and analysis time over existing methods, which use microbiological techniques.
本文描述了一种通过液相色谱结合香草醛柱后衍生化来检测和定量鸡组织中莫能菌素的方法。莫能菌素通过与甲醇 - 水匀浆从组织中提取出来，并通过液 - 液分配和硅胶吸附剂萃取进行分离和浓缩。莫能菌素在酸性条件下于柱后与香草醛混合并加热，然后用在520nm波长下操作的可变波长检测器测量生成的产物。该方法的定量限为0.025微克/克，并已验证可用于分析鸡肌肉、肝脏和皮肤（带有附着脂肪组织）中的莫能菌素。在3个水平上对3种组织类型进行的标准回收率测试范围为82%至96%。与使用微生物技术的现有方法相比，该方法在特异性、准确性和分析时间方面都有改进。
