通过新型克隆速激肽肽受体的异源表达进行功能表征
Functional characterization by heterologous expression of a novel cloned tachykinin peptide receptor.
作者信息
Donaldson L F, Haskell C A, Hanley M R
机构信息
Department of Biological Chemistry, University of California at Davis Medical School 95616, USA.
出版信息
Biochem J. 1996 Nov 15;320 ( Pt 1)(Pt 1):1-5. doi: 10.1042/bj3200001.
Abstract
An orphan receptor resembling the neurokinin 3 tachykinin receptor (NK3), initially claimed to be an atypical opioid receptor, is shown herein to respond potently to the physiological NK3 receptor ligand, neurokinin B. This 'NK4' receptor did not give functional responses in Xenopus oocytes to opioid agonists. However, NK4 receptor activation was inhibited by nanomolar concentrations of dynorphin. The NK4 receptor is therefore a tachykinin receptor which is functionally antagonized by an endogenous opioid peptide.
摘要
一种类似于神经激肽3速激肽受体（NK3）的孤儿受体，最初被认为是一种非典型阿片受体，本文显示它对生理性NK3受体配体神经激肽B有强烈反应。这种“NK4”受体在非洲爪蟾卵母细胞中对阿片类激动剂没有功能性反应。然而，纳摩尔浓度的强啡肽可抑制NK4受体的激活。因此，NK4受体是一种速激肽受体，可被内源性阿片肽进行功能拮抗。
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