Park W Y, Kim J I, Shim E H, Lee W H, Kim S H, Seo J W, Jang J J, Seo J S
Transgenic Mice Center, Seoul National University College of Medicine, Korea.
Cancer Lett. 1996 Oct 22;107(2):293-300. doi: 10.1016/0304-3835(96)04413-8.
We produced transgenic mice using SV40 Tag gene under the control of its own enhancer and promoter. Three transgenic lines (SNU-SVT125, 127, 248) consistently developed thymic carcinoma as well as choroid plexus carcinoma and dysplastic renal tubule. In SNU-SVT248 line, SV40 Tag transgene was expressed at thymus, spleen and kidney. Thymic epithelium showed high level expression of SV40 Tag in immunohistochemistry. Histopathological and electron microscopic analysis revealed that poorly differentiated carcinoma was derived from type 2 to 4 thymic epithelial cell. Our transgenic mice would provide a model for studies on the pathogenesis of thymic carcinoma and on the regulation of thymopoiesis by epithelial cells.
我们利用在其自身增强子和启动子控制下的SV40 Tag基因培育出了转基因小鼠。三个转基因品系（SNU - SVT125、127、248）持续发生胸腺肿瘤以及脉络丛癌和发育异常的肾小管。在SNU - SVT248品系中，SV40 Tag转基因在胸腺、脾脏和肾脏中表达。免疫组织化学显示胸腺上皮呈现SV40 Tag的高水平表达。组织病理学和电子显微镜分析表明，低分化癌源自2型至4型胸腺上皮细胞。我们的转基因小鼠将为胸腺肿瘤发病机制以及上皮细胞对胸腺生成的调节研究提供一个模型。
