Niki I, Yokokura H, Sudo T, Kato M, Hidaka H
Department of Pharmacology, Nagoya University School of Medicine.
J Biochem. 1996 Oct;120(4):685-98. doi: 10.1093/oxfordjournals.jbchem.a021466.
Changes in cytosolic Ca2+ concentrations evoke a wide range of cellular responses and intracellular Ca(2+)-binding proteins are the key molecules to transduce Ca2+ signaling via enzymatic reactions or modulation of protein/protein interations (Fig.1). The EF hand proteins, like calmodulin and S100 proteins, are considered to exert Ca(2+)-dependent actions in the nucleus or the cytoplasm. The Ca2+/phospholipid binding proteins are classified into two groups, the annexins and the C2 region proteins. These proteins, distributed mainly in the cytoplasm, translocate to the plasma membrane in response to an increase in cytosolic Ca2+ and function in the vicinity of the membrane. Ca2+ storage proteins in the endoplasmic or sarcoplasmic reticulum provide the high Ca2+ capacity of the Ca2+ store sites, which regulate intracellular Ca2+ distribution. The variety and complexity of Ca2+ signaling result from the cooperative actions of specific Ca(2+)-binding proteins. This review describes biochemical properties of intracellular Ca(2+)-binding proteins and their proposed roles in mediating Ca2+ signaling.
胞质Ca2+浓度的变化引发了广泛的细胞反应，细胞内Ca(2+)结合蛋白是通过酶促反应或调节蛋白质/蛋白质相互作用来转导Ca2+信号的关键分子（图1）。EF手型蛋白，如钙调蛋白和S100蛋白，被认为在细胞核或细胞质中发挥Ca(2+)依赖性作用。Ca2+/磷脂结合蛋白分为两组，即膜联蛋白和C2区域蛋白。这些蛋白主要分布在细胞质中，响应胞质Ca2+的增加而转位到质膜，并在膜附近发挥作用。内质网或肌浆网中的Ca2+储存蛋白提供了Ca2+储存位点的高Ca2+容量，从而调节细胞内Ca2+分布。Ca2+信号的多样性和复杂性源于特定Ca(2+)结合蛋白的协同作用。本综述描述了细胞内Ca(2+)结合蛋白的生化特性及其在介导Ca2+信号中的作用。
