Tsuruwaka Yusuke, Konishi Masataka, Shimada Eriko
Marine Bioresource Exploration Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC) , Yokosuka , Japan.
School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST) , Nomi, Ishikawa , Japan.
PeerJ. 2015 Jan 27;3:e727. doi: 10.7717/peerj.727. eCollection 2015.
We investigated the role of the WW domain-containing oxidoreductase (wwox) gene in the embryonic development of zebrafish, with particular emphasis on intracellular Ca(2+) dynamics because Ca(2+) is an important intracellular messenger. Comparisons between zebrafish wwox and human WWOX sequences identified highly conserved domain structures. wwox was expressed in developing heart tissues in the zebrafish embryo. Moreover, wwox knockdown induced pericardial edema with similarities to conditions observed in human breast cancer. The wwox knockdown embryos with the edema died within a week. High Ca(2+) levels were observed at the boundary between the edema and yolk in wwox knockdown embryos.
我们研究了含WW结构域的氧化还原酶（wwox）基因在斑马鱼胚胎发育中的作用，特别关注细胞内Ca(2+)动力学，因为Ca(2+)是一种重要的细胞内信使。斑马鱼wwox与人类WWOX序列的比较确定了高度保守的结构域结构。wwox在斑马鱼胚胎发育中的心脏组织中表达。此外，wwox基因敲低诱导心包水肿，类似于在人类乳腺癌中观察到的情况。出现水肿的wwox基因敲低胚胎在一周内死亡。在wwox基因敲低胚胎的水肿和卵黄之间的边界处观察到高Ca(2+)水平。
