Palmiter R D, Mulvihill E R, Shepherd J H, McKnight G S
J Biol Chem. 1981 Aug 10;256(15):7910-6.
Changes in rates of ovalbumin and conalbumin gene transcription and mRNA levels were monitored during an entire cycle of estrogen withdrawal and restimulation. Correlations of transcription rates with nuclear estrogen receptor levels in this experiment and in dose-response experiments reveal that conalbumin gene transcription is directly proportional to nuclear receptor levels, whereas ovalbumin gene transcription is related to receptor levels in a way that suggests cooperative interactions among receptors. Conalbumin mRNA accumulation and transcription are transiently inhibited by administration of progesterone to estrogen-stimulated chicks, whereas ovalbumin gene transcription is stimulated by this regimen. This puzzling observation can be rationalized if a single receptor binding site is involved in conalbumin gene regulation and multiple sites are involved in ovalbumin gene regulation. These ideas are combined with our recent observations that protein synthesis inhibitors and butyrate selectively, but reversibly, inhibit ovalbumin and conalbumin gene transcription. We describe a new hypothesis of how steroid hormones regulate egg white gene transcription in the chick oviduct.
在雌激素撤除和重新刺激的整个周期中，监测了卵清蛋白和伴清蛋白基因转录速率及mRNA水平的变化。在本实验以及剂量反应实验中，转录速率与核雌激素受体水平的相关性表明，伴清蛋白基因转录与核受体水平成正比，而卵清蛋白基因转录与受体水平的关系表明受体之间存在协同相互作用。给雌激素刺激的小鸡注射孕酮会短暂抑制伴清蛋白mRNA的积累和转录，而该方案会刺激卵清蛋白基因转录。如果伴清蛋白基因调控涉及单个受体结合位点，而卵清蛋白基因调控涉及多个位点，那么这一令人困惑的观察结果就可以得到合理的解释。这些观点与我们最近的观察结果相结合，即蛋白质合成抑制剂和丁酸盐选择性但可逆地抑制卵清蛋白和伴清蛋白基因转录。我们描述了一种关于类固醇激素如何调节小鸡输卵管中蛋清基因转录的新假说。
