Mohanan P V, Rathinam K
Toxicology Group, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Thiruvananthapuram, India.
Vet Hum Toxicol. 1996 Dec;38(6):427-8.
The present study evaluated the induction of micronuclear potency of indigenously developed bone wax saline extract. The experiment was designed with 2 batches, each consisting of 8 groups with 3 male Swiss albino mice each. In each batch, the first 3 groups received Chitra's bone wax saline extract and the second 3 groups received Johnson & Johnson bone wax saline extract at 12.5, 25.0 or 50.0 ml/kg body weight ip for 2 d. The remaining 2 groups were vehicle (saline) and positive (cyclophosphamide) controls. Animals were sacrificed 24 and 36 h after the second dosing and bone marrow smears were prepared and evaluated for micronuclei. The Chitra's and Johnson & Johnson's bone wax extracts failed to induce micronuclei in bone marrow erythrocytes of mice.
本研究评估了国产骨蜡盐水提取物诱导微核的能力。实验设计为2个批次，每个批次由8组组成，每组有3只雄性瑞士白化小鼠。在每个批次中，前3组分别以12.5、25.0或50.0 ml/kg体重腹腔注射Chitra骨蜡盐水提取物，后3组分别以相同剂量腹腔注射强生骨蜡盐水提取物，连续注射2天。其余2组分别为溶剂（盐水）对照组和阳性（环磷酰胺）对照组。在第二次给药后24小时和36小时处死动物，制备骨髓涂片并评估微核情况。Chitra和强生的骨蜡提取物均未在小鼠骨髓红细胞中诱导出微核。
