Lutje V, Taylor K A, Kennedy D, Authié E, Boulangé A, Gettinby G
International Livestock Research Institute, Nairobi, Kenya.
Exp Parasitol. 1996 Dec;84(3):320-9. doi: 10.1006/expr.1996.0120.
A comparison of T-cell-mediated immune responses in trypanotolerant N'Dama and susceptible Boran cattle during primary infection with tsetse-transmitted Trypanosoma congolense was conducted to assess whether different patterns of T-cell activation occurred during trypanosome infection. Proliferation and IFN-gamma synthesis in response to trypanosome antigens and to the mitogen Con A were measured in LNC before infection and 10 and 35 days postinfection. Phenotypic analysis of LNC was also carried out. No significant differences in the in vitro proliferation of LNC to VSG, to hsp70/BiP, or to Con A were detected between the breeds. In contrast, IFN-gamma production in response to Con A was higher in Boran cattle at 35 days p.i. A reduction in the number of CD2+ and CD4+ T-cells and an increase in the percentage of B-cells, CD8+ T-cells, and gamma delta T-cells during infection in both N'Dama and Boran was revealed by cytofluorimetric analysis of lymph node cells.
在采采蝇传播的刚果锥虫初次感染期间，对耐锥虫的恩达马牛和易感的博拉纳牛的T细胞介导的免疫反应进行了比较，以评估在锥虫感染期间是否发生了不同模式的T细胞激活。在感染前以及感染后10天和35天，测量了外周血淋巴细胞（LNC）对锥虫抗原和丝裂原刀豆蛋白A（Con A）的增殖反应以及γ干扰素合成。还对外周血淋巴细胞进行了表型分析。两个品种之间，外周血淋巴细胞对变异表面糖蛋白（VSG）、热休克蛋白70/免疫球蛋白结合蛋白（hsp70/BiP）或刀豆蛋白A的体外增殖没有显著差异。相比之下，在感染后35天，博拉纳牛对刀豆蛋白A的γ干扰素产生更高。通过对淋巴结细胞的细胞荧光分析发现，在恩达马牛和博拉纳牛感染期间，CD2⁺和CD4⁺ T细胞数量减少，B细胞、CD8⁺ T细胞和γδ T细胞百分比增加。
