Lutje V, Mertens B, Boulangé A, Williams D J, Authié E
International Livestock Research Institute (ILRI), Nairobi, Kenya.
Exp Parasitol. 1995 Sep;81(2):154-64. doi: 10.1006/expr.1995.1104.
T-cell-mediated immune responses to defined antigens of Trypanosoma congolense were measured in cattle undergoing primary infection. The antigens used were the variable surface glycoprotein and two invariant antigens, a 33-kDa cysteine protease (congopain) and a recombinant form of a 69-kDa heat-shock protein. Proliferative responses were highest during the second week postinfection and were detected in cells obtained from the lymph node draining the site of infection but not in peripheral blood mononuclear cells. Production of IL-2 and IFN-gamma was measured in supernatants from antigen-stimulated lymph node cell cultures. Expression of IL-2, IL-4, and IFN-gamma mRNA was detected in antigen-stimulated lymph node cells by reverse transcription-polymerase chain amplification.
在初次感染的牛中检测了针对刚果锥虫特定抗原的T细胞介导的免疫反应。所用抗原为可变表面糖蛋白和两种不变抗原，一种33 kDa的半胱氨酸蛋白酶（刚果蛋白酶）和一种重组形式的69 kDa热休克蛋白。增殖反应在感染后第二周最高，在从感染部位引流的淋巴结获得的细胞中检测到，但在外周血单核细胞中未检测到。在抗原刺激的淋巴结细胞培养上清液中测量IL-2和IFN-γ的产生。通过逆转录-聚合酶链扩增在抗原刺激的淋巴结细胞中检测IL-2、IL-4和IFN-γ mRNA的表达。
