Stone K E, Sakaguchi D S
Department of Zoology and Genetics, Signal Transduction Training Group, Iowa State University, Ames 50011, USA.
Dev Biol. 1996 Nov 25;180(1):297-310. doi: 10.1006/dbio.1996.0302.
We have examined the function of beta1 integrin receptors and N-cadherin in the development of the Xenopus retinotectal projection. In vivo perturbation experiments were performed by injecting antibodies directed against beta1 integrin receptors and N-cadherin into the embryonic optic pathway. The antibodies were present during the initial development of the retinal projection, when the axons of the ganglion cells are migrating through the optic tract and terminating within the optic tectum. When injected individually, the antibodies were insufficient to cause obvious pathfinding errors. However, when injected together, the antibodies caused specific abnormalities in the development of the retinotectal projection. Pathfinding errors most commonly observed included ectopically projecting axons within the optic tract region, meandering and splaying of axons in the optic tectum, and the induction of prominent ipsilateral projections. IgGs and Fab' fragments of the antibodies produced pathfinding errors; these defects were not observed in animals injected with control antibodies. These in vivo results show that beta1 integrin receptors and N-cadherin have important roles during the development of the visual projection and provide evidence that a balance between cell-cell and cell-matrix adhesion may be critical for the normal development of the vertebrate visual system.
我们研究了β1整合素受体和N-钙黏蛋白在非洲爪蟾视网膜顶盖投射发育中的作用。通过向胚胎视觉通路注射针对β1整合素受体和N-钙黏蛋白的抗体进行体内扰动实验。抗体在视网膜投射的初始发育阶段存在，此时神经节细胞的轴突正在穿过视束并在视顶盖内终止。单独注射时，抗体不足以导致明显的路径寻找错误。然而，一起注射时，抗体在视网膜顶盖投射的发育中导致了特定异常。最常观察到的路径寻找错误包括视束区域内轴突异位投射、视顶盖内轴突蜿蜒和散开，以及诱导明显的同侧投射。抗体的IgG和Fab'片段产生了路径寻找错误；在注射对照抗体的动物中未观察到这些缺陷。这些体内结果表明，β1整合素受体和N-钙黏蛋白在视觉投射发育过程中具有重要作用，并提供了证据表明细胞间和细胞与基质黏附之间的平衡可能对脊椎动物视觉系统的正常发育至关重要。
