Löf K, Lindros K, Seppa K, Fukunaga T, Badger T, Ronis M, Sillanaukee P
Biomedical Research Center, Alko Ltd, Helsinki, Finland.
Alcohol Alcohol. 1996 Sep;31(5):445-51. doi: 10.1093/oxfordjournals.alcalc.a008178.
Serum carbohydrate-deficient transferrin (CDT) is being increasingly used as a biological indicator for excessive alcohol consumption. However, the mechanisms behind the changes in the carbohydrate moiety of transferrin are unclear, although they have been suggested to be mediated by acetaldehyde or liver damage. To study this, an animal model involving alterations in serum isotransferrin concentrations would be needed. The present work examined the changes in the carbohydrate moiety of transferrin in rats after different degrees of ethanol exposure, the effects of chronically elevated acetaldehyde levels, and also the changes, produced with liver toxins (galactosamine) and carbon tetrachloride). Ethanol was administered both in the drinking fluid and by intubation, reaching a dose of 11 g/kg/day over 7 weeks, or 16 g/kg/day over 4 weeks. Serum samples from rats maintained on high ethanol for 10 weeks by intragastric infusion were also analysed. Some rats simultaneously had cyanamide administered to elevate acetaldehyde levels. However, neither ethanol nor acetaldehyde had any effect on transferrin. Intraperitoneal galactosamine, but not carbon tetrachloride, induced transferrin desialylation. Thus, in the rat, neither chronic ethanol consumption nor elevated acetaldehyde induces changes in transferrin microheterogeneity.
血清缺糖转铁蛋白（CDT）正越来越多地被用作过度饮酒的生物学指标。然而，转铁蛋白糖部分变化背后的机制尚不清楚，尽管有人认为其是由乙醛或肝损伤介导的。为了研究这一问题，需要一个涉及血清同种转铁蛋白浓度改变的动物模型。本研究检测了不同程度乙醇暴露后大鼠转铁蛋白糖部分的变化、长期乙醛水平升高的影响，以及肝毒素（半乳糖胺）和四氯化碳所引起的变化。通过在饮水中添加乙醇以及插管给药，7周内达到每日11 g/kg的剂量，或4周内达到每日16 g/kg的剂量。还分析了通过胃内灌注维持高乙醇水平10周的大鼠的血清样本。一些大鼠同时给予氨甲环酸以提高乙醛水平。然而，乙醇和乙醛对转铁蛋白均无任何影响。腹腔注射半乳糖胺而非四氯化碳可诱导转铁蛋白去唾液酸化。因此，在大鼠中，长期饮酒和乙醛水平升高均不会诱导转铁蛋白微异质性的变化。
