非洲爪蟾伴侣蛋白基因Cctg的分子特征分析

Molecular characterisation of the Xenopus laevis chaperonin gene Cctg.

作者信息

Walkley N A, Page R A, Malik A N

机构信息

School of Molecular and Medical Biosciences, University of Wales College of Cardiff, UK.

出版信息

Biochim Biophys Acta. 1996 Nov 11;1309(1-2):25-30. doi: 10.1016/s0167-4781(96)88918-4.

DOI:10.1016/s0167-4781(96)88918-4

PMID:8950171

Abstract

By library screening and PCR we have obtained cDNA clones which encode the gamma subunit of the CCT chaperonin complex from Xenopus laevis. The gene (XlCctg), which encodes the CCT gamma subunit contains an open reading frame which codes for 547 amino acid residues (60 kDa) and the predicted amino acid sequence shares a high degree of sequence identity with other CCT gamma homologues. The XlCctg mRNA measures 2.1 kb and is expressed ubiquitously in all of the X. laevis tissues examined. The mRNA levels of XlCctg are significantly higher in the ovary compared with other tissues.

摘要

通过文库筛选和聚合酶链反应（PCR），我们获得了来自非洲爪蟾（Xenopus laevis）的编码伴侣蛋白CCT复合体γ亚基的cDNA克隆。编码CCTγ亚基的基因（XlCctg）包含一个开放阅读框，该开放阅读框编码547个氨基酸残基（60 kDa），预测的氨基酸序列与其他CCTγ同源物具有高度的序列同一性。XlCctg mRNA大小为2.1 kb，并在所检测的所有非洲爪蟾组织中普遍表达。与其他组织相比，XlCctg在卵巢中的mRNA水平显著更高。