Kuwana H, Shimizu-Nishikawa K, Iwahana H, Yamamoto D
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Biochim Biophys Acta. 1996 Nov 11;1309(1-2):47-52. doi: 10.1016/s0167-4781(96)00137-6.
A novel member of the ATP-binding cassette (ABC) transporter proteins has been cloned from Drosophila melanogaster. The gene is designated as ABC Transporter Expressed in Trachea (Atet), because the transcript was localized to the respiratory system by in situ hybridization analysis of whole-mount embryos using digoxigenin-labeled RNA probes. The hybridization signal was also observed in amnioserosa. Northern blot analysis identified a single 4.5 kb mRNA expressed in all developmental stages at a relatively constant level. The Atet gene mapped to 24E on the left arm of the second chromosome. The Atet protein shows extensive homology with the Drosophila white gene product, which is reported to form heterodimers with the brown or scarlet gene product to transport guanine or tryptophan into the pigment cells in the compound eye. By analogy, Atet is suggested to be involved in transporting a small molecule after dimerization with a partner protein.
已从黑腹果蝇中克隆出一种新型的ATP结合盒（ABC）转运蛋白成员。该基因被命名为气管中表达的ABC转运蛋白（Atet），因为通过使用地高辛标记的RNA探针进行全胚胎原位杂交分析，发现该转录本定位于呼吸系统。在羊膜浆膜中也观察到杂交信号。Northern印迹分析确定了一个单一的4.5 kb mRNA，在所有发育阶段均以相对恒定的水平表达。Atet基因定位于第二条染色体左臂的24E处。Atet蛋白与果蝇白色基因产物具有广泛的同源性，据报道，白色基因产物与棕色或猩红色基因产物形成异二聚体，将鸟嘌呤或色氨酸转运到复眼中的色素细胞中。据此推测，Atet在与伴侣蛋白二聚化后参与小分子的转运。
