2-氯-4-甲硫基丁酸在胃癌致癌物快速生物测定中的作用
Effect of 2-chloro-4-methylthiobutanoic acid in a rapid bioassay for gastric carcinogens.
作者信息
Furihata C, Oka M, Amin S, Krzeminski J, Weisburger J H, Kobayashi K, Tatematsu M
机构信息
Department of Molecular Oncology, Institute of Medical Science, University of Tokyo,
出版信息
Cancer Lett. 1996 Nov 12;108(1):129-35. doi: 10.1016/s0304-3835(96)04404-7.
2-Chloro-4-methylthiobutanoic acid (CMBA, a mutagen from Japanese salted fish) at 15-500 mg/kg body weight induced several-fold increase in replicative DNA synthesis (RDS) (P < 0.05) after 80 min and 17 h, equivocal unscheduled DNA synthesis (UDS) after 80 min and necrosis 80 min after its administration in the stomach pyloric mucosa of F344 and ACI male rats. A positive control, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 50 mg/kg body weight), induced RDS, UDS and erosion. However, the negative control L-methionine (500 mg/kg body weight) did not display any effect. The results suggest possible tumor-initiating and -promoting activity of CMBA but at a lower potency than that of MNNG.
2-氯-4-甲硫基丁酸（CMBA，一种源自日本咸鱼的诱变剂），以15 - 500毫克/千克体重的剂量，在80分钟和17小时后使F344和ACI雄性大鼠胃幽门黏膜中的复制性DNA合成（RDS）增加了数倍（P < 0.05），80分钟时非预定DNA合成（UDS）结果不明确，给药80分钟后出现坏死。阳性对照物N-甲基-N'-硝基-N-亚硝基胍（MNNG，50毫克/千克体重）诱导了RDS、UDS和糜烂。然而，阴性对照物L-甲硫氨酸（500毫克/千克体重）未显示任何作用。结果表明CMBA可能具有肿瘤起始和促进活性，但效力低于MNNG。
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