Yanagida M, Gohda E, Yamamoto I
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Life Sci. 1996;59(24):2075-81. doi: 10.1016/s0024-3205(96)00561-9.
In murine splenocytes, a primary antigen-specific antibody response is stimulated by 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G), a stable form of ascorbate, as described in our previous paper. We examined here the effect of nerve growth factor (NGF) on the antigen-specific antibody production in vitro augmented by AA-2G. NGF (> or = 10 ng/ml), which alone had no effect, enhanced the anti-sheep-red-blood-cell (SRBC) antibody response stimulated by AA-2G. The effect of NGF plus AA-2G or AA-2G alone was abrogated by the presence of castanospermine, an alpha-glucosidase inhibitor, suggesting that the active form of AA-2G is ascorbate. The repeated additions; but not one addition, of ascorbate also resulted in a synergism with NGF on the antibody production. These results suggest that NGF might be a cytokine which functions as a regulatory factor for ascorbate-dependent immune responses.
如我们之前的论文所述，在鼠脾细胞中，一种稳定形式的抗坏血酸盐，即2-O-α-D-吡喃葡萄糖基-L-抗坏血酸（AA-2G），可刺激初次抗原特异性抗体反应。我们在此研究了神经生长因子（NGF）对由AA-2G增强的体外抗原特异性抗体产生的影响。单独使用时无作用的NGF（≥10 ng/ml）增强了由AA-2G刺激的抗绵羊红细胞（SRBC）抗体反应。α-葡萄糖苷酶抑制剂栗精胺的存在消除了NGF加AA-2G或单独使用AA-2G的作用，这表明AA-2G的活性形式是抗坏血酸盐。重复添加抗坏血酸盐（而非单次添加）也导致与NGF在抗体产生上具有协同作用。这些结果表明，NGF可能是一种细胞因子，作为抗坏血酸盐依赖性免疫反应的调节因子发挥作用。
