Funaki N O, Tanaka J, Kasamatsu T, Ohshio G, Hosotani R, Okino T, Imamura M
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Life Sci. 1996;59(25-26):2187-99. doi: 10.1016/s0024-3205(96)00576-0.
To detect adenocarcinoma cells in the circulating peripheral blood, we "analyzed the presence of carcinoembryonic antigen (CEA) mRNA in the peripheral blood obtained from patients with pancreatic carcinoma (PC) or with gastric carcinoma (GC) and also, as controls, from pancreatitis or gastritis patients without carcinomas, a gastric lymphoma patient and four healthy volunteers. Because of the small number of carcinoma cells expected in the peripheral blood, the analysis was performed by the reverse transcription followed by an original two-step polymerase chain reaction. By this sensitive method, 3 of 9 PC patients and 2 of 9 GC patients were positive for CEA mRNA. Except for 1 highly advanced PC patient, 3 of 4 CEA mRNA-positive patients developed recurrence after curative resection or liver metastasis after palliative operation within 9 months after the analysis. None of the control patients was positive for CEA mRNA in the peripheral blood. The results suggest that our sensitive RT-PCR method for detecting CEA mRNA in the peripheral blood is practically useful to find the hematogenous spreading of adenocarcinoma cells.
为了检测循环外周血中的腺癌细胞，我们分析了胰腺癌（PC）或胃癌（GC）患者外周血中癌胚抗原（CEA）mRNA的存在情况，同时，作为对照，也分析了胰腺炎或胃炎患者（无癌症）、一名胃淋巴瘤患者和四名健康志愿者外周血中CEA mRNA的存在情况。由于外周血中预期的癌细胞数量较少，因此通过逆转录随后进行原始的两步聚合酶链反应来进行分析。通过这种灵敏的方法，9例PC患者中有3例、9例GC患者中有2例CEA mRNA呈阳性。除1例高度进展期PC患者外，4例CEA mRNA阳性患者中有3例在分析后9个月内根治性切除后复发或姑息性手术后发生肝转移。对照患者外周血中CEA mRNA均为阴性。结果表明，我们用于检测外周血中CEA mRNA的灵敏逆转录-聚合酶链反应（RT-PCR）方法在实际中有助于发现腺癌细胞的血行播散。
