白内障患者房水中次黄嘌呤和黄嘌呤的水平。

Hypoxanthine and xanthine levels in human aqueous humor from cataractous eyes.

作者信息

Spoto G, Mastropasqua L, Gizzi F, Arduini A, Del Galio P, Ciancaglini M, Gallenga P E, Giacomello A

机构信息

Institute of Biochemical Science, University G.D'Annunzio, Chieti, Italy.

出版信息

Life Sci. 1996;59(25-26):PL387-90. doi: 10.1016/s0024-3205(96)00579-6.

DOI:10.1016/s0024-3205(96)00579-6

PMID:8950326

Abstract

Purine nucleotide degradation products have been determined by HPLC in aqueous humor obtained during cataract surgery and from plasma of 22 patients (12 women). Uric acid, cytosine, guanosine monophosphate, uracyl, guanine, adenosine, adenosine monophosphate, thymine, adenine, inosine, cyclic guanosine monophosphate, hypoxanthine and xanthine were evaluated. Uric acid and the last two were the only compounds detectable in measurable amounts in aqueous humor and in plasma of all patients. Aqueous humor xanthine levels were not significantly different from plasma; aqueous humor hypoxanthine concentrations were lower than those of xanthine and than plasma oxypurine levels. In 8 patients, treated with allopurinol, oxypurinol concentrations in aqueous humor and in plasma were comparable suggesting that oxypurines are transported through the blood-aqueous humor barrier.

摘要

通过高效液相色谱法测定了22例患者（12名女性）白内障手术中获取的房水以及血浆中的嘌呤核苷酸降解产物。对尿酸、胞嘧啶、鸟苷单磷酸、尿嘧啶、鸟嘌呤、腺苷、腺苷单磷酸、胸腺嘧啶、腺嘌呤、肌苷、环鸟苷单磷酸、次黄嘌呤和黄嘌呤进行了评估。尿酸以及最后两种物质是所有患者房水和血浆中仅能检测到的可测量含量的化合物。房水黄嘌呤水平与血浆无显著差异；房水次黄嘌呤浓度低于黄嘌呤浓度以及血浆氧化嘌呤水平。在8例接受别嘌醇治疗的患者中，房水和血浆中的氧嘌呤醇浓度相当，这表明氧化嘌呤可通过血-房水屏障转运。