Cumberbatch M, Dearman R J, Kimber I
Zeneca Central Toxicology Laboratory, Macclesfield, Cheshire, UK.
Arch Dermatol Res. 1996 Nov;288(12):739-44. doi: 10.1007/BF02505290.
The migration of epidermal Langerhans cells (LC) and their transport of antigen from the skin to draining lymph nodes are of considerable importance in the induction of cutaneous immune responses, including contact sensitization. While in transit to the lymph nodes, LC are subject to a number of phenotypic changes required for their movement from the skin and acquisition of the capacity for antigen presentation. Among these are alterations in the expression of adhesion molecules that regulate interactions with the surrounding tissue matrix and with T lymphocytes. In the study described here, we investigated, using a combination of immunocytochemistry and flow cytometry, the expression by LC, and the lymph node dendritic cells into which they mature, of the three adhesion molecules, E-cadherin, intercellular adhesion molecule-1 (ICAM-1) and the membrane glycoprotein CD44. The migration of LC was associated with a marked reduction in the expression of E-cadherin, but a parallel upregulation of ICAM-1. No change in the expression of CD44 was detectable. The significance of these changes and their relevance for the functional maturation of LC are discussed.
表皮朗格汉斯细胞（LC）的迁移及其将抗原从皮肤转运至引流淋巴结的过程，在包括接触性致敏在内的皮肤免疫反应诱导中具有相当重要的意义。在向淋巴结迁移的过程中，LC会经历一些表型变化，这些变化是它们从皮肤移动并获得抗原呈递能力所必需的。其中包括调节与周围组织基质和T淋巴细胞相互作用的黏附分子表达的改变。在本研究中，我们结合免疫细胞化学和流式细胞术，研究了LC及其成熟后形成的淋巴结树突状细胞中三种黏附分子E-钙黏蛋白、细胞间黏附分子-1（ICAM-1）和膜糖蛋白CD44的表达情况。LC的迁移与E-钙黏蛋白表达的显著降低相关，但ICAM-1表达同时上调。未检测到CD44表达的变化。本文讨论了这些变化的意义及其与LC功能成熟的相关性。
