Bedel-Cloutour C H, Mauclaire L, Saux A, Pereyre M
Laboratoire de Chimie Organique et Organométallique, URA 35, Université Bordeaux I, Talence, France.
Bioconjug Chem. 1996 Nov-Dec;7(6):617-27. doi: 10.1021/bc960045i.
The syntheses and characterizations of five differently hydrosoluble monosubstituted aryl porphyrins are reported. Their metallation with indium-111 was achieved and provided tracers with strong specific activities. The covalent coupling between indium-111 porphyrins and BSA served as a model reaction for the definition of the best experimental coupling conditions; the transposition to the labeling of anti-CEA monoclonal antibody was realized. The conjugates exhibited an in vitro good mAb-labeling efficiency, as well as a good preservation of immunoreactivity.
报道了五种不同水溶性单取代芳基卟啉的合成与表征。实现了它们与铟 - 111的金属化，得到了具有高比活度的示踪剂。铟 - 111卟啉与牛血清白蛋白（BSA）之间的共价偶联作为确定最佳实验偶联条件的模型反应；实现了向抗癌胚抗原（CEA）单克隆抗体标记的转化。偶联物在体外表现出良好的单克隆抗体标记效率以及免疫反应性的良好保留。
