Bedel-Cloutour C H, Maneta-Peyret L, Pereyre M, Bezian J H
Laboratoire de Chimie Organique et Organométallique, URA 35 CNRS, Université Bordeaux I, Talence, France.
J Immunol Methods. 1991 Nov 5;144(1):35-41. doi: 10.1016/0022-1759(91)90227-7.
Antibodies were labelled with indium-111 with a view to their use in the radio-immunodetection of cancers. The covalent coupling between indium-111 porphyrin and monoclonal antibodies (IgG and F(ab')2 fragment) was achieved using the ester activated method [N-hydroxy-succinimide/1-ethyl-3-(3-dimethylaminopropyl)carbodiimide]. After purification, this provided conjugated with specific activities of 6 muCi/micrograms Mab (9.3 molecules per Mab) or 1 muCi/microgram (F(ab')2 fragment (1.5 molecule per F(ab')2). ELISA procedures suggested the full retention of immunoreactivity by the radiolabelled antibodies.
为了将抗体用于癌症的放射免疫检测，用铟 - 111对其进行标记。使用酯活化法[N - 羟基琥珀酰亚胺/1 - 乙基 - 3 -（3 - 二甲基氨基丙基）碳二亚胺]实现铟 - 111卟啉与单克隆抗体（IgG和F(ab')2片段）之间的共价偶联。纯化后，得到的缀合物的比活度为6 μCi/μg Mab（每个Mab 9.3个分子）或1 μCi/μg（F(ab')2片段，每个F(ab')2 1.5个分子）。酶联免疫吸附测定程序表明放射性标记抗体完全保留了免疫反应性。
