Planz B, Striepecke E, Wolff J M, Effert P, Jakse G, Böcking A
Department of Urology, Technical University of Aachen, Germany.
Gen Diagn Pathol. 1996 Oct;142(2):69-73.
Biopsies from 60 grade-1 urothelial carcinomas and 50 normal bladder mucosae have been investigated by DNA-image-cytometry after enzymatic cell separation to determine the diagnostic sensitivity of DNA-aneuploidy for the identification of low grade urothelial neoplasia. Two different modes to detect DNA-aneuploidy were compared: the conventional application of a threshold at 2.2c for the modal DNA value and the use of the Kolmogoroff-Smirnow (KS) test to compare GO/1 phase fractions of the cells under analysis and the internal reference cells. Whereas the specificity for both types of analysis was 100%, the sentitivity of the latter method was 73.3% compared to 48.3% of the conventional procedure. Therefore, the application of the KS-test for the identification of DNA- stemlines as aneuploid results in an increased detection rate was compared with the conventional threshold procedure. The high prevalence of DNA- stemline aneuploidy even in low grade urothelial cancers may qualify this marker as a possible aid in the cytologic evaluation of urine and bladder washings.
对60例1级尿路上皮癌和50例正常膀胱黏膜进行活检，经酶法细胞分离后采用DNA图像细胞术进行研究，以确定DNA非整倍体对低级别尿路上皮肿瘤的诊断敏感性。比较了两种检测DNA非整倍体的不同方法：常规应用2.2c作为DNA模式值的阈值，以及使用Kolmogoroff-Smirnow（KS）检验来比较分析细胞与内部参照细胞的G0/1期比例。两种分析类型的特异性均为100%，后一种方法的敏感性为73.3%，而常规方法为48.3%。因此，将KS检验用于识别DNA干系为非整倍体从而提高检测率的方法与常规阈值法进行了比较。即使在低级别尿路上皮癌中DNA干系非整倍体的高发生率，也可能使该标志物成为尿液和膀胱冲洗液细胞学评估的一种可能辅助手段。
