Ciuffetti G, Lombardini R, Pasqualini L, Vaudo G
Sezione di Medicina Interna, Angiologia e Malattie da Arteriosclerosi, Università degli Studi di Perugia.
Ann Ital Med Int. 1996 Jul-Sep;11(3):170-4.
Forearm blood samples were drawn from 18 patients with critical limb ischaemia (stage IIIb peripheral arterial obliterative disease) and 18 age- and sex-matched controls at 8.00 a.m., 2.00 p.m. and 8.00 p.m. We monitored the total and differential leucocyte counts and leucocyte expression of CD11/CD18 integrins (on whole blood samples by indirect immunofluorescence on a FACScan flow cytometer). Our results show no significant difference in any parameter at any stage of the day. At all study times, the total and differential leucocyte counts and granulocyte expression of CD11/CD18 were significantly (p < 0.001) greater in patients compared with controls. Associated with a high leucocyte count, constant up-regulation appears to be a feature of chronic tissue ischaemia. Activated neutrophils seem to aggravate peripheral arterial obliterative disease locally and may contribute to disseminated organ damage.
在上午8点、下午2点和晚上8点，从18例严重肢体缺血（IIIb期外周动脉闭塞性疾病）患者以及18例年龄和性别匹配的对照者身上采集前臂血样。我们监测了白细胞总数、分类计数以及CD11/CD18整合素的白细胞表达（通过在FACScan流式细胞仪上对全血样本进行间接免疫荧光法检测）。我们的结果显示，一天中任何阶段的任何参数均无显著差异。在所有研究时间点，患者的白细胞总数、分类计数以及CD11/CD18的粒细胞表达均显著高于对照组（p < 0.001）。与高白细胞计数相关，持续上调似乎是慢性组织缺血的一个特征。活化的中性粒细胞似乎会局部加重外周动脉闭塞性疾病，并可能导致全身性器官损伤。
