Ciuffetti G, Lombardini R, Paltriccia R, Santambrogio L, Mannarino E
Department of Clinical Medicine, Pathology and Pharmacology, University of Perugia, Italy.
Eur J Clin Invest. 1994 Jan;24(1):65-8. doi: 10.1111/j.1365-2362.1994.tb02061.x.
This ex vivo study determined the expression of leucocyte adhesion receptors for endothelial adhesion molecules in 10 patients with peripheral arterial occlusive disease (PAOD) and in 10 healthy controls before and after treadmill exercise. Granulocytes from venous blood samples were separated on a Ficoll-Hypaque gradient and the phenotypical expression of CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) was observed by double indirect immunofluorescence using specific monoclonal antibodies. The total and differential white blood cell counts were monitored before and after exercise. In the PAOD patients a significant reduction in the number of granulocytes expressing CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) associated with a significant neutropenia was observed after exercise, suggesting that leucocyte-endothelial interactions occur during ischaemia.
这项体外研究测定了10例外周动脉闭塞性疾病（PAOD）患者和10名健康对照者在跑步机运动前后内皮黏附分子的白细胞黏附受体表达情况。通过Ficoll-泛影葡胺梯度分离静脉血样本中的粒细胞，使用特异性单克隆抗体通过双间接免疫荧光观察CD11a/CD18（淋巴细胞功能相关抗原-1）、CD11b/CD18（巨噬细胞-1抗原）和CD11c/CD18（p150,95）的表型表达。在运动前后监测白细胞总数和分类计数。在PAOD患者中，运动后观察到表达CD11b/CD18（巨噬细胞-1抗原）和CD11c/CD18（p150,95）的粒细胞数量显著减少，并伴有明显的中性粒细胞减少，提示在缺血期间发生白细胞与内皮的相互作用。
